Middlebrooks B L, Voss P G, Douglas W L, Toom P M
Department of Biological Sciences, University of Southern Mississippi, Hattiesburg.
J Immunoassay. 1991;12(1):125-44. doi: 10.1080/01971529108055061.
Monoclonal antibodies for use in a ligand displacement assay were selected for specificity and affinity/avidity properties that result in their release and displacement in the presence of specific sample antibody but not in the presence of antibodies against other antigens. A screening process is described which involves measurement of displacement of antibody by an ELISA procedure using an enzyme labeled anti-immunoglobulin, providing a means of demonstrating usefulness of a candidate antibody in a ligand displacement format without necessitating the production of enzyme conjugates of each candidate antibody to be screened. The procedure was used to screen a set of eleven monoclonal antibodies (initially selected for anti-Trichinella spiralis specificity by conventional screening methods), and successfully discriminated between antibodies which were useful in the ligand displacement format and those which were not.
