Smith H J, Snowdon K E
Agriculture Canada, Health of Animals Laboratory, Sackville, New Brunswick.
Can J Vet Res. 1989 Oct;53(4):497-9.
Enzyme immunoassays using the triple antibody enzyme linked immunosorbent assay (ELISA) with both Trichinella spiralis spiralis and T. spiralis nativa excretory-secretory (ES) antigens and a commercial Trichinella spiralis enzyme immunoassay test kit were carried out on sera from pigs that were infected with light, moderate and high doses of infective T. spiralis spiralis and T. spiralis nativa respectively. Seroconversion occurred in all pigs given infective Trichinella larvae although no trichinae were recovered from pigs given T. spiralis nativa larvae and examined between days 92 and 99 postinfection by pepsin digestion. Anti-Trichinella antibodies were detected in pigs infected with T. spiralis spiralis and T. spiralis nativa by ELISA using either the homologous or heterologous ES antigen. The commercial Trichinella spiralis enzyme immunoassay test kit also detected anti-Trichinella antibodies in both the T. spiralis spiralis and T. spiralis nativa infected pigs. The commercial test kit did not appear to be as sensitive as the triple antibody ELISA since it usually took two to three days longer for seroconversion to be detected by the former procedure. Finally seroconversion occurred more rapidly in swine infected with T. spiralis spiralis than with pigs receiving comparable doses of T. spiralis nativa.
采用三联抗体酶联免疫吸附测定（ELISA），使用旋毛形线虫和本地毛形线虫的排泄分泌（ES）抗原，以及商用旋毛虫酶免疫测定试剂盒，对分别感染低、中、高剂量感染性旋毛形线虫和本地毛形线虫的猪血清进行检测。所有接种感染性旋毛虫幼虫的猪均出现血清转化，尽管在感染后92至99天通过胃蛋白酶消化法检查时，接种本地毛形线虫幼虫的猪未检获旋毛虫。使用同源或异源ES抗原，通过ELISA在感染旋毛形线虫和本地毛形线虫的猪中检测到抗旋毛虫抗体。商用旋毛虫酶免疫测定试剂盒也在感染旋毛形线虫和本地毛形线虫的猪中检测到抗旋毛虫抗体。商用试剂盒似乎不如三联抗体ELISA灵敏，因为通常前者检测到血清转化的时间要长两到三天。最后，感染旋毛形线虫的猪比接受相当剂量本地毛形线虫的猪血清转化更快。
