Department of Tropical Diseases and Imaging Diagnosis, Botucatu Medical School, UNESP - Univ. Estadual Paulista, São Paulo, Brazil.
Chemotherapy. 2010;56(2):112-9. doi: 10.1159/000313523. Epub 2010 Apr 20.
Oxacillin is the main drug used for the treatment of Staphylococcus aureus infections. However, resistance of S. aureus to oxacillin has become a major problem over recent decades. The aim of this study was to determine oxacillin resistance in S. aureus isolates obtained from the University Hospital of the Botucatu Medical School, Universidade Estadual Paulista, Brazil.
A total of 102 isolates collected between 2002 and 2006 were analyzed by detection of the mecA gene, cefoxitin and oxacillin disk diffusion methods, screening test on Mueller-Hinton agar and E-test.
Forty-six (45.1%) isolates were mecA positive. The oxacillin disk diffusion method showed 86.9% sensitivity and 91.1% specificity. The screening method and cefoxitin disk diffusion presented a similar sensitivity (91.3%) and the same specificity. The E-test showed 97.8% sensitivity and the same specificity as observed for the other methods.
The E-test yielded the best results compared to the other methods.
苯唑西林是治疗金黄色葡萄球菌感染的主要药物。然而，近年来金黄色葡萄球菌对苯唑西林的耐药性已成为一个主要问题。本研究旨在确定巴西圣保罗州立大学博图卡图医学院附属医院分离的金黄色葡萄球菌中苯唑西林耐药情况。
对 2002 年至 2006 年间收集的 102 株分离株进行 mecA 基因检测、头孢西丁和苯唑西林纸片扩散法、Mueller-Hinton 琼脂筛选试验和 E 试验。
46 株（45.1%）分离株 mecA 阳性。苯唑西林纸片扩散法的敏感性为 86.9%，特异性为 91.1%。筛选法和头孢西丁纸片扩散法的敏感性相似（91.3%），特异性相同。E 试验的敏感性为 97.8%，与其他方法的特异性相同。
与其他方法相比，E 试验的结果最佳。
