Lee Ji-Yeon, Choi Jeong-Sun, Choi Jae-Youn, Shin Yoo-Jin, Yun Hou, Cha Jung-Ho, Chun Myung-Hoon, Lee Mun-Yong
Department of Anatomy, College of Medicine, The Catholic University of Korea, Seoul, Korea.
Acta Neurobiol Exp (Wars). 2010;70(1):1-12. doi: 10.55782/ane-2010-1768.
We have examined the temporal changes and cellular localization of osteopontin (OPN) mRNA and protein in organotypic hippocampal slice cultures subjected to ischemia-like oxygen-glucose deprivation (OGD). The sequential induction pattern response consisted of neuronal and microglial OPN upregulation, followed by a later extended phase of expression in reactive astrocytes. OPN immunoreactivity after OGD matched the mRNA induction patterns. Activated microglia revealed OPN staining in focal deposits, whereas neurons and reactive astrocytes showed perinuclear staining with a punctate cytosolic pattern of OPN, typical of secreted proteins. These data demonstrated that the temporal and cellular patterns of OPN induction in reactive glial cells in this in vitro model closely correlated with that in the in vivo model, suggesting that OPN has a multifunctional role in the pathogenesis of ischemic injury.
我们研究了在经历类似缺血的氧糖剥夺（OGD）的器官型海马切片培养物中骨桥蛋白（OPN）mRNA和蛋白质的时间变化及细胞定位。顺序诱导模式反应包括神经元和小胶质细胞中OPN上调，随后是反应性星形胶质细胞中较晚且持续时间更长的表达阶段。OGD后的OPN免疫反应性与mRNA诱导模式相符。活化的小胶质细胞在局灶性沉积物中显示出OPN染色，而神经元和反应性星形胶质细胞则显示出核周染色，并伴有OPN典型的点状胞质模式，这是分泌蛋白的特征。这些数据表明，在该体外模型中，反应性胶质细胞中OPN诱导的时间和细胞模式与体内模型密切相关，提示OPN在缺血性损伤的发病机制中具有多功能作用。
