Ojcius D M, Zheng L M, Sphicas E C, Zychlinsky A, Young J D
Laboratory of Cellular Physiology and Immunology, Rockefeller University, New York, NY 10021.
J Immunol. 1991 Jun 15;146(12):4427-32.
CTL, NK cells, and lymphokine-activated killer (LAK) cells are cytolytic lymphocytes known to produce a pore-forming protein, named perforin or cytolysin, that lyses target cells by forming large pores on the plasma membrane of the target cell. Other proteins besides perforin are found in the cytoplasmic granules of effector lymphocytes, and these include a family of serine esterases. Ultrastructural immunogold labeling studies with antibodies against perforin and a serine esterase (MTSP-1, also known as granzyme A and SE-1) show that all the granules of LAK cells and a CTL cell line contain perforin and serine esterase. For both LAK cells and CTL, perforin has been located mostly in the fine granular matrix of the granules, whereas gold particles corresponding to serine esterase have been found in both the matrix and the cap regions of the granules. Results from double immunogold labeling indicate that perforin and serine esterase colocalize to the same granules.
细胞毒性T淋巴细胞（CTL）、自然杀伤（NK）细胞和淋巴因子激活的杀伤（LAK）细胞是已知能产生一种名为穿孔素或溶细胞素的成孔蛋白的溶细胞性淋巴细胞，该蛋白通过在靶细胞的质膜上形成大孔来裂解靶细胞。效应淋巴细胞的细胞质颗粒中除了穿孔素外还发现了其他蛋白质，其中包括一个丝氨酸酯酶家族。用抗穿孔素和一种丝氨酸酯酶（MTSP - 1，也称为颗粒酶A和SE - 1）的抗体进行的超微结构免疫金标记研究表明，LAK细胞和一个CTL细胞系的所有颗粒都含有穿孔素和丝氨酸酯酶。对于LAK细胞和CTL来说，穿孔素大多位于颗粒的细颗粒基质中，而与丝氨酸酯酶相对应的金颗粒则在颗粒的基质和帽状区域都有发现。双重免疫金标记的结果表明穿孔素和丝氨酸酯酶共定位于相同的颗粒中。
