Groscurth P, Qiao B Y, Podack E R, Hengartner H
J Immunol. 1987 May 1;138(9):2749-52.
The localization of perforin 1 (P1) in cytotoxic cells was studied by immuno-electron microscopy by using a monospecific rabbit antiserum against highly purified mouse P1 and protein A gold as a second ligand. P1 was found in specific granules of cloned cytotoxic T lymphocytes (CTL). Within the granules, P1 antigen was localized in the fine granular matrix, whereas the vesicular compartment remained free of gold particles. The amount of P1 antigen detectable by immuno-electron microscopy varied between different CTL clones. CTL with NK-like activity had the highest level of P1 antigen. A cytotoxicity loss CTL mutant had no detectable P1 antigen, suggesting an important role of P1 during cell-mediated cytolysis. P1 antigen was undetectable also in bone marrow macrophages, indicating a different cytolytic mechanism of these cells.
利用针对高度纯化的小鼠穿孔素1（P1）的单特异性兔抗血清以及作为第二配体的蛋白A金，通过免疫电子显微镜研究了穿孔素1（P1）在细胞毒性细胞中的定位。在克隆的细胞毒性T淋巴细胞（CTL）的特异性颗粒中发现了P1。在颗粒内，P1抗原定位于细颗粒基质中，而泡状区室没有金颗粒。通过免疫电子显微镜可检测到的P1抗原量在不同的CTL克隆之间有所不同。具有NK样活性的CTL的P1抗原水平最高。一个细胞毒性丧失的CTL突变体没有可检测到的P1抗原，这表明P1在细胞介导的细胞溶解过程中起重要作用。在骨髓巨噬细胞中也检测不到P1抗原，这表明这些细胞具有不同的细胞溶解机制。
