Fereidouni Sasan R, Grund Christian, Häuslaigner Rafaela, Lange Elke, Wilking Hendrik, Harder Timm C, Beer Martin, Starick Elke
Institute of Diagnostic Virology, Friedrich-Loeffler-Institut, Südufer 10, 17493 Greifswald, Insel Riems, Germany.
Avian Dis. 2010 Mar;54(1):79-85. doi: 10.1637/9005-073109-Reg.1.
Natural infections with different subtypes of low pathogenicity avian influenza viruses (LPAIVs) are very common in wild duck populations. Recent outbreaks of high pathogenicity avian influenza virus (HPAIV) H5N1 in Eurasian and African countries stimulated monitoring activities in aquatic wild bird populations. Surveillance mainly focused on virus detection. Only a few serologic investigations have been conducted so far, although such data may retrospectively elucidate epidemiologic patterns of different AIV subtypes in the populations under study. To better understand the immunologic and serologic reactions of mallards after infection with LPAIV, we investigated the AIV type- and subtype-specific antibody dynamics in mallards after different LPAIV infections by hemagglutination inhibition, competitive enzyme-linked immunosorbent assay, and western blot analysis, as appropriate. Four groups of mallard ducks were used: 1) naturally infected birds, 2) birds that were experimentally infected with LPAIV, 3) birds that were immunized with inactivated virus preparations, and 4) negative control birds. Ducks were monitored for up to 15 mo, and serum samples were investigated every 1-4 wk. It could be shown that infection with LPAIV in mallards can be traced serologically over prolonged periods of time.
低致病性禽流感病毒（LPAIV）不同亚型的自然感染在野鸭种群中非常普遍。近期欧亚和非洲国家高致病性禽流感病毒（HPAIV）H5N1的暴发促使人们对野生水鸟种群展开监测活动。监测主要集中在病毒检测上。尽管此类数据可能有助于追溯所研究种群中不同禽流感病毒亚型的流行病学模式，但到目前为止仅进行了少数血清学调查。为了更好地了解野鸭感染LPAIV后的免疫和血清学反应，我们通过血凝抑制试验、竞争酶联免疫吸附测定以及必要时的蛋白质印迹分析，研究了不同LPAIV感染后野鸭体内禽流感病毒型别和亚型特异性抗体的动态变化。使用了四组野鸭：1）自然感染的鸟类，2）经实验感染LPAIV的鸟类，3）用灭活病毒制剂免疫的鸟类，4）阴性对照鸟类。对野鸭进行了长达15个月的监测，每1 - 4周采集血清样本进行检测。结果表明，通过血清学方法可以在很长一段时间内追踪野鸭感染LPAIV的情况。
