Institute of Materials Research and Engineering, Agency for Science, Technology and Research (A*STAR), 3 Research Link, Singapore 117602, Singapore.
Biointerphases. 2008 Sep;3(3):66-74. doi: 10.1116/1.2965133.
The conformational changes in elastinlike polypeptides (ELPs) grafted to a solid/solution interface via different architectures were studied using surface plasmon resonance spectroscopy and surface plasmon field-enhanced fluorescence spectroscopy (SPFS). SPFS provides a simple and convenient optical method to study the influence of the grafting method and the graft density on the conformational changes in ELPs at the solid-solution interface as a function of environmental variables. A typical response of the ELP, consistent with its stimuli responsiveness, was a gradual collapse upon increasing the ionic strength; this effect was inversely correlated with the surface graft density of the ELP.
采用表面等离子体共振光谱和表面等离子体场增强荧光光谱（SPFS）研究了通过不同结构接枝到固/液界面的弹性蛋白样多肽（ELPs）的构象变化。SPFS 提供了一种简单方便的光学方法，可研究接枝方法和接枝密度对固/液界面上 ELPs 构象变化的影响，以及环境变量的影响。ELP 的典型响应与其刺激响应一致，即在增加离子强度时逐渐崩溃；这种效应与 ELP 的表面接枝密度呈反比。
