School of Materials Science and Engineering, Clemson University, Clemson, South Carolina 29634, USA.
Biointerphases. 2009 Jun;4(2):FA17-21. doi: 10.1116/1.3101907.
The authors described an original approach for a surface protein imprinting employing grafting of polymer brushes. Protein molecules were first chemically bound to an ultrathin (1-3 nm) poly(glycidyl methacrylate) reactive polymer layer and later removed by protease treatment. Residual amino acids became grafted to the surface and to a certain extent imitated the surface chemical composition and shape of the template molecule on a nanolevel. The space surrounding the adsorbed biomolecules was modified with grafted poly(ethylene glycol) layer. This led to the formation of islands of spatial nanosized pockets complementary to the protein shape. The adsorbing protein recognized the surfaces imprinted and was anchored to the substrate.
作者描述了一种采用接枝聚合物刷的表面蛋白印迹的原始方法。首先，蛋白质分子通过化学键合到超薄（1-3nm）聚（甲基丙烯酸缩水甘油酯）反应性聚合物层上，然后通过蛋白酶处理去除。残留的氨基酸被接枝到表面上，并在纳米级程度上在一定程度上模仿了模板分子的表面化学组成和形状。吸附生物分子周围的空间用接枝的聚（乙二醇）层进行修饰。这导致形成与蛋白质形状互补的空间纳米口袋的岛。吸附的蛋白质识别印迹表面并固定在基底上。
