Laboratory of Aquaculture & Artemia Reference Center, Ghent University, Ghent, Belgium.
J Appl Microbiol. 2010 Sep;109(3):1007-16. doi: 10.1111/j.1365-2672.2010.04728.x.
In this study, we investigated the effect of N-acyl homoserine lactone-degrading bacterial enrichment cultures (ECs) on larviculture of the giant freshwater prawn Macrobrachium rosenbergii.
The larval performance in terms of larval growth, larval survival, larval quality, duration of the larval rearing process and microflora levels in the rearing water as well as inside the prawn gut was investigated. The application of the EC bacteria was performed in two ways: by adding them directly into the larval rearing water and via enriched Artemia nauplii used for larval feeding. The results of the study demonstrated that both ECs that were tested had a similar positive effect on larval survival and larval quality, whereas they did not affect larval growth or the duration of the larval rearing process.
Under normal hatchery conditions, the optimal EC densities were found to be 10(6) CFU ml(-1) for adding into the rearing water and 5 × 10(8) CFU ml(-1) for enrichment of Artemia nauplii used for feeding of the larvae. In the hatchery, the ECs can be grown on waste streams of Artemia hatching.
Application of this kind of ECs could lead to a more sustainable aquaculture production, by replacing the use of antibiotics to control diseases.
本研究旨在探讨 N-酰基高丝氨酸内酯降解细菌富集培养物（EC）对罗氏沼虾幼体养殖的影响。
本研究从幼体生长、幼体存活率、幼体质量、幼体养殖过程持续时间以及养殖水和对虾肠道内微生物区系水平等方面考察了 EC 细菌的应用效果。EC 细菌的应用有两种方式：直接添加到幼体养殖水中，以及通过富集的丰年虫用于幼体喂养。研究结果表明，两种测试的 EC 均对幼体存活率和幼体质量有相似的积极影响，而对幼体生长或幼体养殖过程持续时间没有影响。
在正常孵化条件下，添加到养殖水中的最佳 EC 密度为 10（6）CFU ml（-1），用于喂养幼虫的丰年虫的最佳 EC 密度为 5×10（8）CFU ml（-1）。在孵化场中，EC 可以在丰年虫孵化的废水流上生长。
应用这种 EC 可以通过替代抗生素来控制疾病，从而实现更可持续的水产养殖生产。
