SKLLQG, Institute of Earth Environment, Chinese Academy of Science, Xi'an, China.
Photochem Photobiol. 2010 May-Jun;86(3):600-5. doi: 10.1111/j.1751-1097.2010.00723.x. Epub 2010 Feb 11.
To determine the effect of CO(2) laser pretreatment of wheat seeds on the physiological tolerance of seedlings to chilling stress, wheat seeds were exposed to CO(2) laser radiation for 300 s. After being cultivated for 48 h at 25 degrees C, the wheat seedlings were subjected to chilling stress for 24 h. Selected physiological and biochemical parameters were measured in 6-day-old seedlings. We observed that chilling stress enhanced the concentrations of malondialdehyde and oxidized glutathione while decreasing the activities of nitric oxide synthase, catalase, peroxidase, superoxide dismutase and the concentrations of nitric oxide and glutathione in the wheat leaves compared with controls. When the chilling stress was preceded by CO(2) laser irradiation, the concentrations of malondialdehyde and oxidized glutathione were decreased while the activities of nitric oxide synthase, catalase, peroxidase, superoxide dismutase and the concentrations of nitric oxide and glutathione increased. Furthermore, chilling stress decreased the biomass, biophoton intensity and GHS/GSSG ratios of seedlings while these parameters increased when the seedlings were treated with CO(2) laser irradiation prior to the chilling stress. The results suggest that a suitable dose of CO(2) laser stimulation can enhance the physiological tolerance of wheat seedlings to chilling stress.
为了确定 CO2 激光预处理小麦种子对幼苗抗冷胁迫生理耐受性的影响，将小麦种子暴露于 CO2 激光辐射 300 秒。在 25°C 下培养 48 小时后，将小麦幼苗置于冷胁迫下 24 小时。在 6 天大的幼苗中测量了选定的生理生化参数。我们观察到，与对照相比，冷胁迫增强了丙二醛和氧化型谷胱甘肽的浓度，同时降低了一氧化氮合酶、过氧化氢酶、过氧化物酶、超氧化物歧化酶的活性以及一氧化氮和谷胱甘肽的浓度。当冷胁迫前进行 CO2 激光辐照时，丙二醛和氧化型谷胱甘肽的浓度降低，而一氧化氮合酶、过氧化氢酶、过氧化物酶、超氧化物歧化酶的活性以及一氧化氮和谷胱甘肽的浓度增加。此外，冷胁迫降低了幼苗的生物量、生物光子强度和 GHS/GSSG 比值，而当幼苗在冷胁迫前用 CO2 激光处理时，这些参数增加。结果表明，适当剂量的 CO2 激光刺激可以增强小麦幼苗对冷胁迫的生理耐受性。
