Esim Nevzat, Atici Okkes, Mutlu Salih
1Vocational Training School, Bingol University, Bingöl, Turkey.
Toxicol Ind Health. 2014 Apr;30(3):268-74. doi: 10.1177/0748233712457444. Epub 2012 Aug 17.
The effects of nitric oxide (NO) on chilling tolerance (contents of hydrogen peroxide (H2O2) and superoxide anion (O2 (-)) and lipid peroxidation level (malondialdehyde, MDA)) and the activities of antioxidant enzymes (superoxide dismutase (SOD), peroxidase (POX) and catalase (CAT)) were investigated in the leaves of wheat (Triticum aestivum L.) exposed to chilling. NO treatment was carried out through spraying of sodium nitroprusside (SNP), which is a donor of NO. To do this, SNP concentrations of 0.1 and 1 mM were applied on the leaves of 11-day plants and the plants were then exposed to chilling conditions (5/2°C) for 3 days. The chilling stress treatment increased both the activities of antioxidant enzymes and the levels of MDA, H2O2 and O2 (-). Similarly, NO treatment enhanced SOD, POX and CAT activities under chilling stress, whereas it decreased H2O2 and O2 (.) (-) contents as well as MDA level. The most effective concentration was determined as 0.1 mM SNP. Exogenous SNP application as a donor of NO was found to have an important ameliorative effect on cold tolerance of seedling exposed to chilling stress by stimulating antioxidant enzyme activity.
研究了一氧化氮（NO）对低温胁迫下小麦（Triticum aestivum L.）叶片抗冷性（过氧化氢（H2O2）和超氧阴离子（O2 (-)）含量以及脂质过氧化水平（丙二醛，MDA））和抗氧化酶（超氧化物歧化酶（SOD）、过氧化物酶（POX）和过氧化氢酶（CAT））活性的影响。通过喷施硝普钠（SNP，一种NO供体）进行NO处理。为此，将0.1 mM和1 mM的SNP浓度施用于11日龄植株的叶片上，然后将植株置于低温条件（5/2°C）下处理3天。低温胁迫处理增加了抗氧化酶的活性以及MDA、H2O2和O2 (-)的水平。同样，NO处理增强了低温胁迫下SOD、POX和CAT的活性，同时降低了H2O2和O2 (.) (-)的含量以及MDA水平。最有效的浓度确定为0.1 mM SNP。发现外源施用SNP作为NO供体，通过刺激抗氧化酶活性，对遭受低温胁迫的幼苗的抗冷性具有重要的改善作用。
