Key Laboratory of Urban Environment and Health, Institute of Urban Environment, Chinese Academy of Sciences, Xiamen, People's Republic of China.
Bioresour Technol. 2010 Aug;101(16):6309-14. doi: 10.1016/j.biortech.2010.03.040. Epub 2010 Apr 20.
In this study, a lab-scale sequencing batch biofilm reactor (SBBR) was used to start-up the completely autotrophic nitrogen removal over nitrite (Canon) process from conventional activated sludge under salt stress for 118days. A persistent, stable partial nitrification was achieved in the SBBR when the salt concentration gradually increased to 6.5 g NaCl L(-1). Anaerobic ammonium oxidation (Anammox) bacteria were successfully enriched from the conventional activated sludge in the SBBR after 68 days of operation. Anammox bacterial strains similar to the order Planctomycetales, genus Candidatus brocardia and genus Candidatus kuenenia were confirmed to exist in the internal layer of the biofilm via Fluorescence in Situ Hybridization (FISH) analysis. A maximal total nitrogen (TN) removal rate of 0.072 kg N m(-3) d(-1) was achieved for the Canon process when the salinity was maintained at a constant 10.0 g NaCl L(-1) in the SBBR. This reactor may have applications for the Canon process treatment of salinity wastewaters containing high concentrations of ammonia.
在这项研究中，使用实验室规模的序批式生物膜反应器（SBBR），在盐胁迫下，经过 118 天，使传统活性污泥从常规活性污泥中完全自养脱氮（CANON）过程启动。当盐浓度逐渐增加到 6.5gNaCl/L 时，SBBR 中实现了持续稳定的部分硝化。经过 68 天的运行，厌氧氨氧化（Anammox）细菌成功地从 SBBR 中的传统活性污泥中富集。通过荧光原位杂交（FISH）分析，确认在生物膜的内层存在类似于浮霉菌门、布鲁氏菌属和卡登尼亚菌属的 Anammox 细菌株。当 SBBR 中的盐度保持在 10.0gNaCl/L 时，CANON 过程的最大总氮（TN）去除率达到 0.072kgN/m3/d。该反应器可能适用于处理含有高浓度氨的含盐废水的 CANON 工艺。
