胃癌和胃上皮异型增生作为癌前病变中的线粒体微卫星不稳定。

Mitochondrial microsatellite instability in gastric cancer and gastric epithelial dysplasia as a precancerous lesion.

机构信息

Department of Surgery, Keimyung University College of Medicine, 2800 Dalgubeoldaero, Dalseo-Gu, Daegu, Republic of Korea.

出版信息

Cancer Epidemiol. 2010 Jun;34(3):323-7. doi: 10.1016/j.canep.2010.03.015. Epub 2010 Apr 20.

DOI:10.1016/j.canep.2010.03.015

PMID:20409774

Abstract

BACKGROUND

Genetic instability in gastric cancer represents a key molecular step that occurs early in the carcinogenesis process. To clarify the role of genetic instability in the progression from gastric dysplasia to gastric cancer, mitochondrial microsatellite instability (mtMSI) was studied in gastric cancer and gastric dysplasia.

METHODS

DNA was isolated from paired normal and tumoral tissues in 24 patients with gastric dysplasia (low grade) and 49 patients with gastric cancer. mtMSI was analyzed using eight microsatellite markers. mtMSI in gastric dysplasia was studied prospectively to elucidate the relation between mtMSI and gastric carcinogenesis.

RESULTS

mtMSI was found in 5 (10.2%) of 49 gastric cancer patients. The mtMSI phenotype was not associated with age, gender, and Helicobacter pylori infection. However, all of the mtMSI was found in intestinal-type gastric cancer (20.8%, p=0.02). In gastric dysplasia, mtMSI was detected in 3 (12.5%) of 24 patients with gastric dysplasia. mtMSI-positive gastric dysplasia showed a poor prognosis statistically compared to mtMSI negative through progression to high-grade dysplasia or gastric cancer.

CONCLUSIONS

These data suggest that mtMSI may be an early and important event in the progression of gastric carcinogenesis, especially in intestinal-type gastric cancer.

摘要

背景

胃癌中的遗传不稳定性代表了在癌变过程中早期发生的关键分子步骤。为了阐明遗传不稳定性在从胃发育不良到胃癌的进展中的作用，研究了胃癌和胃发育不良中的线粒体微卫星不稳定性（mtMSI）。

方法

从 24 例低级别胃发育不良患者和 49 例胃癌患者的配对正常和肿瘤组织中分离 DNA。使用八个微卫星标记物分析 mtMSI。前瞻性研究胃发育不良中的 mtMSI，以阐明 mtMSI 与胃癌发生之间的关系。

结果

在 49 例胃癌患者中发现 mtMSI 存在于 5 例（10.2％）中。mtMSI 表型与年龄、性别和幽门螺杆菌感染无关。然而，所有 mtMSI 均存在于肠型胃癌中（20.8％，p=0.02）。在胃发育不良中，在 24 例胃发育不良患者中的 3 例（12.5％）中检测到 mtMSI。与 mtMSI 阴性相比，mtMSI 阳性的胃发育不良通过进展为高级别发育不良或胃癌在统计学上显示出较差的预后。