Department of Oral and Maxillofacial Surgery, Christian-Albrechts University Kiel, Kiel, Germany.
Int J Oral Maxillofac Surg. 2010 Jul;39(7):699-704. doi: 10.1016/j.ijom.2010.03.016. Epub 2010 Apr 21.
The aim of this study was to compare vacuum and conventional dressings and to follow revascularization with laser-Doppler spectroscopy and histological evaluations. In six minipigs, full thickness skin grafts were harvested on both sides of the back and transplanted to the contralateral site. One site was covered with a classical cotton dressing, the other with a vacuum dressing. For 10 days, oxygen, haemoglobin, flow and velocity levels were measured by laser-Doppler spectroscopy. Data were analyzed with ANOVA. Histological evaluation included haematoxylin and eosin (H&E) as well as CD31 immunohistochemical staining. Oxygen levels were significantly reduced in the vacuum dressing group compared with the classic dressing group during the first 3 days after transplantation. Haemoglobin levels were slightly, but significantly, higher in the vacuum dressing group over the whole observation period. On the second and third day after transplantation single capillaries were detectable in the histological evaluation. Starting from the fourth day, capillary number increased. Similar results for the classical pressure dressing and the vacuum dressing were observed. The present experimental model provides a standardized and reliable test system for evaluating revascularization of full thickness skin grafts in conjunction with growth factors and other enhancers of revascularization.
本研究旨在比较真空敷料和传统敷料，并通过激光多普勒光谱和组织学评估来跟踪再血管化情况。在六头小型猪的背部两侧采集全厚皮片，并将其移植到对侧部位。一侧用经典的棉敷料覆盖，另一侧用真空敷料覆盖。在 10 天的时间里，通过激光多普勒光谱测量氧、血红蛋白、流量和速度水平。数据采用 ANOVA 进行分析。组织学评估包括苏木精和伊红（H&E）染色以及 CD31 免疫组织化学染色。与传统敷料组相比，移植后前 3 天，真空敷料组的氧水平明显降低。在整个观察期间，真空敷料组的血红蛋白水平略高，但有显著差异。在移植后的第二天和第三天，在组织学评估中可以检测到单个毛细血管。从第四天开始，毛细血管数量增加。经典压力敷料和真空敷料的结果相似。本实验模型提供了一个标准化和可靠的测试系统，可用于评估与生长因子和其他再血管化增强剂联合使用的全厚皮片的再血管化情况。
