Biomedical Engineering Group, Department of Applied Mechanics, Indian Institute of Technology Madras, Chennai 600036, India.
J Biophotonics. 2011 Jan;4(1-2):92-7. doi: 10.1002/jbio.200900096. Epub 2010 Apr 22.
In biomedical optical spectroscopy tissue-mimicking phantoms have been widely used for imitating optical properties of biological tissues. As tissue is a turbid medium involving scatterers, absorbing and fluorescing molecules, modelling a tissue in the form of a phantom should have the same realistic complexity comparable to that of tissues. In optical spectroscopy, fluorescence phenomena have been extensively investigated as an optical technique for disease diagnosis. The fluorescence signal is distorted by optical properties of a biological tissue. The purpose of this study is to investigate whether the use of Intralipid as a scattering agent in a turbid medium containing fluorophores can affect fluorescent intensity by the phenomena of scattering and collisional quenching. The results indicate that phantom sets with different concentrations of Tyrosine and Intralipid have their emission peaks distorted at 300 nm and also show secondary peaks when used for fluorescence studies in UV region.
在生物医学光学生物光学光谱学中，组织模拟体被广泛用于模拟生物组织的光学特性。由于组织是一种涉及散射体、吸收和荧光分子的混浊介质，因此以模拟体的形式对组织进行建模应该具有与组织相当的真实复杂性。在光学生物光学中，荧光现象已被广泛研究作为一种用于疾病诊断的光学技术。荧光信号会被生物组织的光学特性所扭曲。本研究旨在探讨在含有荧光团的混浊介质中使用 Intralipid 作为散射剂是否会通过散射和碰撞猝灭现象影响荧光强度。结果表明，在 Tyrosine 和 Intralipid 浓度不同的模拟体中，其在 300nm 处的发射峰发生了扭曲，并且在 UV 区域进行荧光研究时也出现了次峰。
