Department of Food Science and Technology, College of Agricultural and Environmental Sciences, The University of Georgia, 100 Cedar Street, Athens, Georgia 30602, USA.
J Agric Food Chem. 2010 May 26;58(10):6102-9. doi: 10.1021/jf1004836.
The antioxidant and topical anti-inflammatory activities of low and high molecular weight phenolic fractions (LMPF and HMPF, respectively) isolated from three blackberry cultivars (i.e., Navaho, Kiowa, and Ouachita), bred to tolerate the warm and humid climatic conditions of the southeastern United States, were investigated by the in vitro ferric reducing antioxidant power (FRAP) assay and an in vivo mouse ear edema model. Seventy percent (v/v) acidified acetone was employed to extract phenolics from the Georgia-grown blackberry cultivars, which were subsequently cleaned up on an Amberlite XAD-16 column and then further fractionated with Sephadex LH-20 to LMPF and HMPF. The anti-inflammatory response from topical application of solutions of the LMPF and HMPF as well as indomethacin, a potent nonsteroidal anti-inflammatory drug, was assessed in the TPA mouse ear model. All treatments significantly (P < 0.05) reduced TPA-induced irritation injury. Furthermore, mouse ear myeloperoxidase (MPO) activity, an indicator of polymorphonuclear leukocyte infiltration, was assessed and found to be significantly (P < 0.05) reduced after topical application of indomethacin and all blackberry preparations. Correlation coefficients of 0.925 and 0.923 (P < 0.01) were determined when the anti-inflammatory activities of the blackberry fractions were compared to their total phenolics contents and antioxidant activities (i.e., FRAP values), respectively.
从三种黑莓品种（即纳瓦霍、基奥瓦和瓦奇塔）中分离出的低分子量和高分子量酚类化合物（分别为 LMPF 和 HMPF）具有抗氧化和局部抗炎活性，这些黑莓品种经过培育能够耐受美国东南部温暖潮湿的气候条件。通过体外铁还原抗氧化能力（FRAP）测定和体内小鼠耳水肿模型研究了这些活性。采用 70%（v/v）酸化丙酮从佐治亚州种植的黑莓品种中提取酚类化合物，然后用 Amberlite XAD-16 柱进行净化，再用 Sephadex LH-20 进一步分离为 LMPF 和 HMPF。通过 TPA 小鼠耳模型评估了 LMPF 和 HMPF 溶液以及非甾体抗炎药吲哚美辛的局部抗炎反应。所有处理均显著（P < 0.05）减轻了 TPA 诱导的刺激损伤。此外，还评估了小鼠耳髓过氧化物酶（MPO）活性，这是一种多形核白细胞浸润的指标，发现吲哚美辛和所有黑莓制剂的局部应用后，MPO 活性显著（P < 0.05）降低。当将黑莓馏分的抗炎活性与其总酚含量和抗氧化活性（即 FRAP 值）进行比较时，相关性系数分别为 0.925 和 0.923（P < 0.01）。
