刺桐和积雪草叶提取物的抗氧化及抗炎活性比较研究。
Comparative study on anti-oxidant and anti-inflammatory activities of Caesalpinia crista and Centella asiatica leaf extracts.
作者信息
Ramesh B N, Girish T K, Raghavendra R H, Naidu K Akhilender, Rao U J S Prasada, Rao K S
机构信息
Department of Agricultural Biotechnology, Agricultural College, Karekere, Hassan, University of Agricultural Sciences (B), Mysore, Karnataka, India.
Department of Biochemistry and Nutrition, CSIR-Central Food Technological Research Institute, Mysore, Karnataka, India.
出版信息
J Pharm Bioallied Sci. 2014 Apr;6(2):86-91. doi: 10.4103/0975-7406.129172.
BACKGROUND
Amyloidosis, oxidative stress and inflammation have been strongly implicated in neurodegenerative disorders like Alzheimer's disease. Traditionally, Caesalpinia crista and Centella asiatica leaf extracts are used to treat brain related diseases in India. C. crista is used as a mental relaxant drink as well as to treat inflammatory diseases, whereas C. asiatica is reported to be used to enhance memory and to treat dementia.
OBJECTIVE
The present study is aimed to understand the anti-oxidant and anti-inflammatory potential of C. asiatica and C. crista leaf extracts.
MATERIALS AND METHODS
Phenolic acid composition of the aqueous extracts of C. crista and C. asiatica were separated on a reverse phase C18 column (4.6 x 250 mm) using HPLC system. Antioxidant properties of the leaf extracts were determined by 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging assay and the reducing potential assay. The anti-inflammatory activities of aqueous extracts of C. crista and C. asiatica were studied using 5-lipoxygenase assay. Polymorphonuclear leukocytes (PMNLs) were isolated from blood by Ficoll-Histopaque density gradient followed by hypotonic lysis of erythrocytes.
RESULTS
Gallic, protocatechuic, gentisic, chlorogenic, caffeic, p-coumaric and ferulic acids were the phenolic acids identified in C. crista and C. asiatica leaf aqueous extracts. However, gallic acid and ferulic acid contents were much higher in C. crista compared to C. asiatica. Leaf extracts of C. asiatica and C. crista exhibited antioxidant properties and inhibited 5-lipoxygenase (anti-inflammatory) in a dose dependent manner. However, leaf extracts of C. crista had better antioxidant and anti-inflammatory activity compared to that of C. asiatica. The better activity of C. crista is attributed to high gallic acid and ferulic acid compared to C. asiatica.
CONCLUSIONS
Thus, the leaf extract of C. crista can be a potential therapeutic role for Alzheimer's disease.
背景
淀粉样变性、氧化应激和炎症与阿尔茨海默病等神经退行性疾病密切相关。在印度，传统上使用大托叶云实和积雪草叶提取物来治疗脑部相关疾病。大托叶云实被用作一种精神放松饮品以及治疗炎症性疾病，而积雪草据报道可用于增强记忆力和治疗痴呆症。
目的
本研究旨在了解积雪草和大托叶云实叶提取物的抗氧化和抗炎潜力。
材料与方法
使用高效液相色谱系统在反相C18柱（4.6×250mm）上分离大托叶云实和积雪草水提取物中的酚酸成分。通过1,1-二苯基-2-苦基肼（DPPH）自由基清除试验和还原电位试验测定叶提取物的抗氧化性能。使用5-脂氧合酶试验研究大托叶云实和积雪草水提取物的抗炎活性。通过Ficoll-Histopaque密度梯度从血液中分离多形核白细胞（PMNLs），随后对红细胞进行低渗裂解。
结果
没食子酸、原儿茶酸、龙胆酸、绿原酸、咖啡酸、对香豆酸和阿魏酸是在大托叶云实和积雪草叶水提取物中鉴定出的酚酸。然而，大托叶云实中的没食子酸和阿魏酸含量比积雪草中的高得多。积雪草和大托叶云实的叶提取物表现出抗氧化性能，并以剂量依赖方式抑制5-脂氧合酶（抗炎）。然而，大托叶云实的叶提取物比积雪草具有更好的抗氧化和抗炎活性。大托叶云实的更好活性归因于其比积雪草含有更高的没食子酸和阿魏酸。
结论
因此，大托叶云实叶提取物可能对阿尔茨海默病具有潜在的治疗作用。
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