Bettin Pires Larissa, Giuliano-Caetano Lucia, Dias Ana Lúcia
Universidade Estadual de Londrina, CCB, Departamento de Biologia Geral, 86051-990, Londrina, PR, Brasil.
Folia Biol (Krakow). 2010;58(1-2):29-34. doi: 10.3409/fb58_1-2.29-34.
The karyotypes of three species of fish of the Cichlidae family from the Forqueta river and several locations in Guaiba lake/RS (Brazil) were analyzed. All species presented 2n=48, while Gymnogeophagus gymnogenys showed two karyotypic formulae: 4m+44st-a with FN=52 and 6m+42st-a with FN=54. Gymnogeophagus labiatus presented 4m+4sm+40st-a and FN=56 and Geophagus brasiliensis 4sm+44st-a and FN=52. Simple NORs were found in all species with the exception of a population of G. gymnogenys from Saco da Alemoa/Barra do Ribeiro. CMA3 staining revealed NOR sites, while DAPI staining was negative and heterochromatin was limited to pericentromeric regions an d associated to NORs, except in G. labiatus. The data show a conserved pattern in Geophagus brasiliensis and karyotype variation in the species of Gymnogeophagus.
对来自福尔凯塔河以及巴西南里奥格兰德州瓜伊巴湖多个地点的丽鱼科三种鱼类的核型进行了分析。所有物种的二倍体染色体数均为2n = 48，而裸身裸丽鱼呈现出两种核型公式：4m + 44st - a，臂数FN = 52，以及6m + 42st - a，臂数FN = 54。唇裸丽鱼的核型为4m + 4sm + 40st - a，臂数FN = 56，巴西陶乐鲷为4sm + 44st - a，臂数FN = 52。除了来自阿莱莫阿湾/里贝罗河口的一个裸身裸丽鱼种群外，在所有物种中均发现了简单的核仁组织区（NORs）。CMA3染色显示了NOR位点，而DAPI染色为阴性，异染色质仅限于着丝粒周围区域并与NORs相关，唇裸丽鱼除外。数据表明巴西陶乐鲷具有保守的核型模式，而裸丽鱼属物种存在核型变异。
