Departament de Bioquímica i Biologia Molecular, Facultat de Biologia, Universitat de Barcelona, Avda. Diagonal 645, 08028 Barcelona, Spain.
J Physiol Biochem. 2010 Mar;66(1):55-62. doi: 10.1007/s13105-010-0009-7. Epub 2010 Apr 27.
The role of ErbB4 and ErbB2 in the heart of adult mammals is well established. The heart also expresses ErbB1 (the epidermal growth factor (EGF) receptor), but this receptor has received less attention. We studied the effect of EGF on the response of isolated mouse heart to low-flow ischemia and reperfusion. Reducing perfusate flow to 10% for 30 min resulted in an increase in anaerobic metabolism and the leakage of lactate dehydrogenase during reperfusion. In addition, left ventricle +dP/dt and developed pressure were depressed (20-25%) during reperfusion. The addition of EGF 5 min before and throughout the ischemic period prevented the increase in anaerobic metabolism and the leakage of intracellular lactate dehydrogenase during reperfusion. EGF improved both +dP/dt and developed pressure during ischemia and prevented the decrease in dP/dt during reperfusion. To determine whether the effect of EGF on cell integrity depends on its effect on contractility, we studied nonbeating isolated myocytes. In these cells, anoxia and reoxygenation reduced cell viability by nearly 25%. EGF prevented such a decrease. Our results indicate that, like ErbB4 and ErbB2, ErbB1 also has an important role in the heart of adult animals.
在成年哺乳动物的心脏中，ErbB4 和 ErbB2 的作用已得到充分证实。心脏还表达 ErbB1（表皮生长因子 (EGF) 受体），但该受体受到的关注较少。我们研究了 EGF 对离体小鼠心脏对低流量缺血再灌注反应的影响。将灌流液流量减少到正常的 10%持续 30 分钟导致在再灌注期间无氧代谢增加和乳酸脱氢酶漏出。此外，左心室+dP/dt 和发展压在再灌注期间（20-25%）受到抑制。在缺血期间，EGF 在再灌注前 5 分钟和整个缺血期间添加可防止无氧代谢增加和细胞内乳酸脱氢酶漏出。EGF 在缺血期间改善+dP/dt 和发展压，并防止再灌注期间 dP/dt 的下降。为了确定 EGF 对细胞完整性的影响是否依赖于其对收缩性的影响，我们研究了非搏动性分离的心肌细胞。在这些细胞中，缺氧和复氧使细胞活力降低了近 25%。EGF 可防止这种下降。我们的结果表明，与 ErbB4 和 ErbB2 一样，ErbB1 在成年动物的心脏中也具有重要作用。
