Xu Jian-hua, Zhang Chao, Tang Bo, Hao Ying-xue, Chen Jun, Liu Tao, Cui Hao
Department of General Surgery, Southwest Hospital, The Third Military Medical University, Chongqing 400038, China.
Zhonghua Wei Chang Wai Ke Za Zhi. 2010 Apr;13(4):282-5.
To investigate the regulatory role of JAK2/STAT3/vimentin signaling pathway on the proliferation and migration of human colon cancer cells.
The human colon cancer cell Lovo was treated with Janus kinase inhibitor AG490. The proliferation of Lovo cells was quantified by MTT assay. The migration of Lovo cells was measured with scratch assay. The intracellular phosphorylation STAT3 (P-STAT3) and vimentin protein was detected by Western blot and immunofluorescence.
After AG490 treatment, the proliferative ability of Lovo cells decreased as compared with control group (P<0.05). This suppression was dose-independent and time-independent. At 24 hrs after scratching, scratch width in the AG490 group recovered to 20%, lower than that in the control group (60%, P<0.05). After AG490 treatment, the expression of P-STAT3 and vimentin in Lovo cells decreased significantly (P<0.05).
JAK2/STAT3/vimentin signaling pathway participates in regulating the proliferation and migration of human colon cancer cells.
探讨JAK2/STAT3/波形蛋白信号通路对人结肠癌细胞增殖和迁移的调控作用。
用Janus激酶抑制剂AG490处理人结肠癌细胞Lovo。采用MTT法检测Lovo细胞的增殖情况。用划痕试验检测Lovo细胞的迁移能力。通过蛋白质免疫印迹法和免疫荧光法检测细胞内磷酸化STAT3（P-STAT3）和波形蛋白的表达。
AG490处理后，Lovo细胞的增殖能力与对照组相比降低（P<0.05）。这种抑制作用与剂量和时间无关。划痕后24小时，AG490组的划痕宽度恢复至20%，低于对照组（60%，P<0.05）。AG490处理后，Lovo细胞中P-STAT3和波形蛋白的表达明显降低（P<0.05）。
JAK2/STAT3/波形蛋白信号通路参与调控人结肠癌细胞的增殖和迁移。
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