Sun Kuan-Xue, Xia Hong-Wei, Xia Rong-Long
Department of General Surgery, Gongli Hospital of Shanghai Pu Dong New District Shanghai 200135, China ; Department of Ultrasound Diagnosis, Gongli Hospital of Shanghai Pu Dong New District Shanghai 200135, China.
Int J Clin Exp Pathol. 2015 Jan 1;8(1):615-21. eCollection 2015.
Salidroside is considered to have anti-tumor properties. We investigate its effects on colon carcinoma SW1116 cells. Cell viability was assessed by CCK-8. Propidium iodide (PI) staining was used to determine the cell cycle by flow cytometry. The migration and invasion were detected by Transwell. Western blot was used to detect the expression of STAT3 signal related proteins. As the result, high concentrations of salidroside (10, 20. 50 μg/ml) significantly inhibited proliferation of SW1116 cells in a parallelly, cell cycle arrest was increased at the G0/G1 phase after salidroside treatment. Furthermore, salidroside inhibited migration and invasion of SW1116 cells. Salidroside treatment decreased proteins expression of phosphorylation levels in JAK2/STAT3 signaling, while MMP-2 and MMP-9 proteins levels were decreased and protein expression of VEGF and VEGFR-2 were down-regulated. In Conclusion, salidroside inhibited proliferation, decreased the migration and invasion of SW1116 cells in JAK2/STAT3-dependent pathway, the specific mechanisms need further study.
红景天苷被认为具有抗肿瘤特性。我们研究了其对结肠癌SW1116细胞的影响。通过CCK-8评估细胞活力。采用碘化丙啶（PI）染色，通过流式细胞术确定细胞周期。通过Transwell检测迁移和侵袭情况。使用蛋白质免疫印迹法检测STAT3信号相关蛋白的表达。结果显示，高浓度的红景天苷（10、20、50μg/ml）能显著抑制SW1116细胞的增殖，且呈平行关系，红景天苷处理后，细胞周期在G0/G1期的阻滞增加。此外，红景天苷抑制SW1116细胞的迁移和侵袭。红景天苷处理降低了JAK2/STAT3信号通路中磷酸化水平的蛋白表达，同时MMP-2和MMP-9蛋白水平降低，VEGF和VEGFR-2的蛋白表达下调。总之，红景天苷在JAK2/STAT3依赖的途径中抑制SW1116细胞的增殖、迁移和侵袭，具体机制有待进一步研究。
