Wang Fei, Jiang Jian-ming, Wang Feng-long, Fu Zhao-zong, Zhang Zhao-fei, Qu Dong-bin
Department of Orthopedic and Spinal Surgery, Nanfang Hospital, Southern Medical University, Guangzhou510515, China.
Nan Fang Yi Ke Da Xue Xue Bao. 2010 Apr;30(4):871-4.
To observe the morphology and phenotypes of cells extracted from the endplate in the intervertebral discs and identify the factors affecting their biological characteristic.
The intervertebral disc endplate were digested enzymatically, and the morphology of the obtained cells was examined under light microscope. Immunhistochemical analysis of collagen II and real-time PCR was carried out, and the morphologies, viability, cell growth, apoptosis and chondrocyte matrix production were compared between the cells isolated from the degenerative and normal vertebral endplates.
The cells in primary culture presented with spherical and oval morphology, and the cytoplasm was stained blue with toluidine blue. The morphologies of the cartilage endplate cells and the articular cells were almost identical. All the freshly isolated cells expressed collagen II. The degenerative vertebral endplate cells showed decreased expression of collagen II with increased apoptotic cells as compared with normal vertebral endplate cells.
The intervertebral disc endplate cells, like articular cartilage cells, express cartilage-specific matrix proteins. Degenerative vertebral endplate cells show decreased cell vitality with increases cell apoptosis.
观察从椎间盘终板提取的细胞的形态和表型，并确定影响其生物学特性的因素。
对椎间盘终板进行酶消化，在光学显微镜下检查所获细胞的形态。进行Ⅱ型胶原免疫组织化学分析和实时聚合酶链反应，并比较从退变和正常椎体终板分离的细胞之间的形态、活力、细胞生长、凋亡及软骨细胞基质产生情况。
原代培养的细胞呈球形和椭圆形，细胞质经甲苯胺蓝染色呈蓝色。软骨终板细胞和关节细胞的形态几乎相同。所有新鲜分离的细胞均表达Ⅱ型胶原。与正常椎体终板细胞相比，退变椎体终板细胞的Ⅱ型胶原表达降低，凋亡细胞增多。
椎间盘终板细胞与关节软骨细胞一样，表达软骨特异性基质蛋白。退变椎体终板细胞活力降低，细胞凋亡增加。
