Chambers P, Matthews D A, Pringle C R, Easton A J
Department of Biological Sciences, University of Warwick, Coventry, U.K.
Virus Res. 1991 Mar;18(2-3):263-70. doi: 10.1016/0168-1702(91)90023-o.
We have cloned eight intergenic regions from the Pneumovirus pneumonia virus of mice that link the nine small and medium sized genes previously described (Chambers et al., 1990). The nucleotide sequences of the clones confirm the locations of these genes and their mRNA transcripts in the viral genome. The intergenic regions vary in size from 2-56 nucleotides and show only faint homology to each other or to their analogues in respiratory syncytial virus. Sequence alignments suggest that the location of the transcriptional start site for the mRNA encoding the major nucleocapsid protein of pneumonia virus of mice and respiratory syncytial virus may have altered during virus evolution by gain or loss of a transcriptional start signal.
我们从小鼠肺炎病毒中克隆了8个基因间隔区，这些间隔区连接了之前描述的9个中小型基因（钱伯斯等人，1990年）。克隆片段的核苷酸序列证实了这些基因及其mRNA转录本在病毒基因组中的位置。基因间隔区的大小从2至56个核苷酸不等，彼此之间或与呼吸道合胞病毒中的类似区域仅有微弱的同源性。序列比对表明，在病毒进化过程中，编码小鼠肺炎病毒和呼吸道合胞病毒主要核衣壳蛋白的mRNA转录起始位点的位置可能因转录起始信号的获得或丢失而发生了改变。
