van Staden Raluca-Ioana Stefan, van Staden Jacobus Frederick, Aboul-Enein Hassan Y, Balcu Ionel
Laboratory of Electrochemistry and PATLAB Bucharest, National Institute of Research for Electrochemistry and Condensed Matter, 202 Splaiul Independentei Str., Bucharest, Romania.
Comb Chem High Throughput Screen. 2010 Jul;13(6):497-501. doi: 10.2174/138620710791516094.
A sequential injection analysis system was selected for the simultaneous determination of L- and D-T4 in raw material and pharmaceutical products, because of the highest precision and accuracy, the lower consumption of sample and buffer, and the flexibility of selecting different buffers accordingly with the type of sensors utilized as detectors. An amperometric biosensor based on D-amino acid oxidase was used as detector for D-T4 and an immunosensor based on anti-L-T4 was used as detector for L-T4. The SIA/biosensors system can be used reliably on-line in synthesis process control, for the simultaneous assay of L- and D- T4 with a frequency of more than 30 samples per hour.
由于具有最高的精密度和准确度、较低的样品和缓冲液消耗量以及根据用作检测器的传感器类型选择不同缓冲液的灵活性，因此选择了顺序注射分析系统来同时测定原料药和药品中的L-T4和D-T4。基于D-氨基酸氧化酶的安培生物传感器用作D-T4的检测器，基于抗L-T4的免疫传感器用作L-T4的检测器。SIA/生物传感器系统可在合成过程控制中可靠地在线使用，以每小时超过30个样品的频率同时测定L-T4和D-T4。
