在不同阶段的假孕兔中，PGF2α给药后黄体雌激素受体、白细胞介素-1 和凋亡相关基因的表达。
Expression of luteal estrogen receptor, interleukin-1, and apoptosis-associated genes after PGF2alpha administration in rabbits at different stages of pseudopregnancy.
机构信息
Department of Veterinary Biopathological Science, Laboratory of Biotechnology, Section of Physiology, University of Perugia, Perugia, Italy.
出版信息
Domest Anim Endocrinol. 2010 Aug;39(2):116-30. doi: 10.1016/j.domaniend.2010.03.001. Epub 2010 Apr 10.
The dynamic expression for estrogen receptor subtype-1 (ESR1), interleukin-1beta (IL1B), and apoptosis-associated genes, as well as nitric oxide synthase activity, were examined in corpora lutea (CL) of rabbits after prostaglandin F(2alpha) (PGF(2alpha)) administration on either day 4 or day 9 of pseudopregnancy. By reverse transcriptase polymerase chain reaction, the steady-state level of ESR1 transcript was lower (P < 0.01) and that of anti-apoptotic B-cell CLL/lymphoma 2 (BCL2) -like 1 (BCL2L1) was greater in day 4 (P < 0.01) than in day 9 CL. Western blot analysis revealed that BCL2-associated X protein (BAX) abundance was greater in day 4 (P < 0.01) than in day 9 CL, whereas BCL2L1 protein was undetectable at both luteal stages. After PGF(2alpha), ESR1 transcript decreased (P < 0.01) in day 9 CL, whereas IL1B mRNA showed a transitory increase (P < 0.01) at both stages. The pro-apoptotic tumor protein p53 (TP53) gene had diminished (P < 0.01) on day 4 and on day 9 after a transitory increase (P < 0.01), whereas the BAX/BCL2L1 expression ratio increased (P < 0.01) in day 9 CL 24 h after treatment. Following PGF(2alpha), TP53 protein increased (P < 0.01) at both luteal stages, and BAX decreased (P < 0.01) in day 4 CL but increased (P < 0.01) 24 h later in day 9 CL; BCL2L1 became detectable 6 h later in day 4 CL. Nitric oxide synthase activity temporarily increased (P < 0.01) following PGF(2alpha). These findings suggest that PGF(2alpha) regulates luteolysis by ESR1 mRNA down-regulation and modulation of pro- and anti-apoptotic pathways in CL that have acquired a luteolytic capacity.
前列腺素 F2α（PGF2α）在假孕第 4 天或第 9 天给药后，检测了兔黄体（CL）中雌激素受体亚型 1（ESR1）、白细胞介素 1β（IL1B）和凋亡相关基因的动态表达以及一氧化氮合酶活性。通过逆转录聚合酶链反应，第 4 天（P <0.01）ESR1 转录本的水平较低，而抗凋亡 B 细胞 CLL/淋巴瘤 2（BCL2）样 1（BCL2L1）的水平较高。Western blot 分析显示，第 4 天（P <0.01）BAX 丰度大于第 9 天 CL，而 BCL2L1 蛋白在两个黄体阶段均不可检测。PGF2α 后，第 9 天 CL 中的 ESR1 转录本减少（P <0.01），而 IL1B mRNA 则在两个阶段均表现出短暂增加（P <0.01）。促凋亡肿瘤蛋白 p53（TP53）基因在第 4 天减少（P <0.01），在第 9 天短暂增加（P <0.01）后减少，而 BAX/BCL2L1 表达比在治疗后第 9 天 CL 增加（P <0.01）24 小时。PGF2α 后，TP53 蛋白在两个黄体阶段均增加（P <0.01），第 4 天 CL 中的 BAX 减少（P <0.01），但在第 9 天 CL 24 小时后增加（P <0.01），BCL2L1 在第 4 天 CL 中 6 小时后变得可检测。PGF2α 后一氧化氮合酶活性暂时增加（P <0.01）。这些发现表明，PGF2α 通过下调 ESR1 mRNA 并调节已获得黄体溶解能力的 CL 中的促凋亡和抗凋亡途径来调节黄体溶解。
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