Abd-Elkareem Mahmoud, Abou-Elhamd Alaa Sayed
Department of Anatomy, Histology and Embryology, Faculty of Veterinary Medicine, Assiut University, Egypt.
Department of Medical Laboratory Technology, Faculty of Medical applied sciences, Jazan University, KSA.
Anim Reprod. 2019 Oct 24;16(2):302-310. doi: 10.21451/1984-3143-AR2018-0128.
Progesterone plays an important role in the reproductive function and follicular development in mammals. The aim of the present study was to examine the localization of progesterone receptor alpha (PRA) in ovary of pseudopregnant rabbit by immunohistochemical methods. Samples were collected from 14 h. to 18 days of pseudopregnancy. At the first stage of pseudopregnancy (14 h.), the rabbit ovary showed moderate immunostaining of PRA in the granulosa cells and theca interna cells of preovulatory follicle and in the stroma cells. At the middle stage of pseudopregnancy (3-7 days), the rabbit ovary showed strong immunostaining of PRA in ovarian surface epithelial cells, follicular cells of the primary follicle, granulosa cells and theca interna cells of the growing and antral follicles. Moderate immunoexpression of PRA were observed in the large lutein cells and endothelial cells of the corpus haemorrhagicum and corpus luteum and in the stroma cells. At the end of pseudopregnancy (18 days) strong PRA reactions were detected in the small lutein cells of the regressed corpus luteum. Moderate to strong PRA immuno-expression were observed in the proliferated theca interna cells of the atretic antral follicles. The atretic large lutein cells of the regressed corpus luteum showed negative immunostaining for PRA. This study showed that the PRA positive small lutein cells of the regressed corpus luteum and the PRA positive proliferated theca interna cells of the atretic antral follicles were transformed into PRA positive interstitial gland cells. In conclusion, the present study had described the distribution of PRA in the ovary of pseudopregnant rabbit, which is not discussed before in the available literature. It also gives more information about follicular dynamic, formation and origin of interstitial glands, mechanism of ovulation, formation and regression of the corpus luteum.
孕酮在哺乳动物的生殖功能和卵泡发育中起着重要作用。本研究的目的是通过免疫组织化学方法检测孕酮受体α（PRA）在假孕兔卵巢中的定位。样本采集于假孕的14小时至18天。在假孕的第一阶段（14小时），兔卵巢在排卵前卵泡的颗粒细胞和内膜细胞以及基质细胞中显示出PRA的中度免疫染色。在假孕的中期（3 - 7天），兔卵巢在卵巢表面上皮细胞、初级卵泡的卵泡细胞、生长卵泡和窦状卵泡的颗粒细胞和内膜细胞中显示出PRA的强免疫染色。在出血性黄体和黄体的大黄体细胞、内皮细胞以及基质细胞中观察到PRA的中度免疫表达。在假孕末期（18天），在退化黄体的小黄体细胞中检测到强烈的PRA反应。在闭锁窦状卵泡增殖的内膜细胞中观察到中度至强烈的PRA免疫表达。退化黄体的闭锁大黄体细胞对PRA呈阴性免疫染色。本研究表明，退化黄体中PRA阳性的小黄体细胞和闭锁窦状卵泡中PRA阳性的增殖内膜细胞转化为PRA阳性的间质腺细胞。总之，本研究描述了PRA在假孕兔卵巢中的分布，这在现有文献中之前未被讨论过。它还提供了更多关于卵泡动态、间质腺的形成和起源、排卵机制、黄体的形成和退化的信息。
