Departamento de Ciencias Básicas, Centro de Investigación de Enfermedades Tropicales.
Mem Inst Oswaldo Cruz. 2010 Mar;105(2):184-90. doi: 10.1590/s0074-02762010000200013.
The chemical composition and biological activities of 19 essential oils and seven of their major components were tested against free and intracellular forms of Leishmania chagasi and Trypanosoma cruzi parasites as well as Vero and THP-1 mammalian cell lines. The essential oils were obtained from different species of Lippia, a widely distributed genus of Colombian plants. They were extracted by microwave radiation-assisted hydro-distillation and characterised by GC-FID and GC-MS. The major components were geranial, neral, limonene, nerol, carvacrol, p-cymene, gamma-terpinene, carvone and thymol. The essential oil of Lippia alba exhibited the highest activity against T. cruzi epimastigotes and intracellular amastigotes with an IC50 of 5.5 microg/mL and 12.2 microg/mL, respectively. The essential oil of Lippia origanoides had an IC50 of 4.4 microg/mL in L. chagasi promastigotes and exhibited no toxicity in mammalian cells. Thymol (IC50 3.2 +/- 0.4 microg/mL) and S-carvone (IC50 6.1 +/- 2.2 microg/mL), two of the major components of the active essential oils, were active on intracellular amastigotes of T. cruziinfected Vero cells, with a selective index greater than 10. None of the essential oils or major components tested in this study was active on amastigotes of L. chagasi infected THP-1 cells.
从广泛分布于哥伦比亚的 Lippia 属的不同植物物种中提取了 19 种精油和其中 7 种主要成分，并对游离和细胞内形式的莱什曼原虫和克氏锥虫寄生虫以及 Vero 和 THP-1 哺乳动物细胞系进行了测试，以检测其化学成分和生物活性。采用微波辐射辅助水蒸馏法提取精油，并通过 GC-FID 和 GC-MS 进行了表征。主要成分有香叶醛、橙花醛、柠檬烯、橙花醇、香芹酚、对伞花烃、γ-萜品烯、香芹酮和百里酚。Lippia alba 的精油对 T. cruzi 滋养体和内阿米巴原虫表现出最高的活性，IC50 分别为 5.5 μg/mL 和 12.2 μg/mL。Lippia origanoides 的精油对 L. chagasi 前鞭毛体的 IC50 为 4.4 μg/mL，对哺乳动物细胞无毒性。两种主要成分百里酚（IC50 3.2 ± 0.4 μg/mL）和 S-香芹酮（IC50 6.1 ± 2.2 μg/mL）对感染 T. cruzi 的 Vero 细胞内阿米巴原虫具有活性，选择性指数大于 10。在本研究中测试的精油或主要成分均对感染 THP-1 细胞的 L. chagasi 内阿米巴原虫没有活性。
