Laboratório de Parasitologia, Centro Acadêmico de Vitória, Universidade Federal de Pernambuco (CAV/UFPE), Vitória de Santo Antão, PE, Brazil.
Laboratório de Biologia Molecular, Departamento de Bioquímica, Universidade Federal de Pernambuco (UFPE), Recife, PE, Brazil.
Arch Microbiol. 2021 Sep;203(7):4303-4311. doi: 10.1007/s00203-021-02377-5. Epub 2021 Jun 10.
The aim of this study was to evaluate the antioxidant, antibacterial, and antibiofilm activities of nerolidol. The antioxidant activity of nerolidol was determined using the total antioxidant activity method. Antibacterial activity was performed using the microdilution method to determine the minimum inhibitory concentration (MIC) against seven standard strains of the ATCC and four bacterial clinical isolates with a resistance profile, following the Clinical and Laboratory Standards Institute (CLSI). The antibiofilm activity of nerolidol was performed using the crystal violet method. The results of the antioxidant test revealed a total antioxidant activity of 93.94%. Nerolidol inhibited the growth of Staphylococcus aureus (MIC = 1 mg/mL), Streptococcus mutans (MIC = 4 mg/mL), Pseudomonas aeruginosa (MIC = 0.5 mg/mL), and Klebsiella pneumoniae (MIC = 0.5 mg/mL). For clinical isolates, nerolidol showed an inhibitory potential against multidrug-resistant P. aeruginosa, K. pneumoniae carbapenemase (MIC = 0.5 mg/mL), methicillin-susceptible S. aureus (MIC = 2 mg/mL), and methicillin-resistant S. aureus (MIC = 2 mg/mL). Nerolidol showed similar antibacterial activity against ATCC strains and hospital clinical isolates with resistance profile, suggesting that even though these strains are resistant to antibiotics, they are still sensitive to nerolidol. Nerolidol exerted a dose-dependent effect on the inhibition of biofilm formation, even at subinhibitory concentrations. Nerolidol inhibited bacterial biofilms of ATCC strains at a rate ranging from 51 to 98%, at concentrations ranging from 0.5 to 4 mg/mL. For clinical bacterial isolates, biofilm inhibition ranged from 6 to 60%. Therefore, the present study showed the antioxidant, antibacterial, and antibiofilm properties of nerolidol.
本研究旨在评估橙花叔醇的抗氧化、抗菌和抗生物膜活性。采用总抗氧化活性法测定橙花叔醇的抗氧化活性。采用微量稀释法测定最低抑菌浓度（MIC），以评估橙花叔醇对 ATCC 标准菌株和具有耐药谱的四种临床分离菌的抗菌活性，该方法遵循临床和实验室标准协会（CLSI）的标准。橙花叔醇的抗生物膜活性采用结晶紫法测定。抗氧化试验结果显示，其总抗氧化活性为 93.94%。橙花叔醇抑制金黄色葡萄球菌（MIC=1mg/mL）、变异链球菌（MIC=4mg/mL）、铜绿假单胞菌（MIC=0.5mg/mL）和肺炎克雷伯菌（MIC=0.5mg/mL）的生长。对于临床分离株，橙花叔醇对多药耐药铜绿假单胞菌、碳青霉烯酶阴性肺炎克雷伯菌（MIC=0.5mg/mL）、甲氧西林敏感金黄色葡萄球菌（MIC=2mg/mL）和耐甲氧西林金黄色葡萄球菌（MIC=2mg/mL）具有抑制潜力。橙花叔醇对 ATCC 菌株和具有耐药谱的医院临床分离株具有相似的抗菌活性，这表明即使这些菌株对抗生素耐药，它们仍然对橙花叔醇敏感。橙花叔醇对生物膜形成的抑制作用呈剂量依赖性，即使在亚抑菌浓度下也是如此。橙花叔醇以 0.5 至 4mg/mL 的浓度，对 ATCC 菌株的细菌生物膜的抑制率为 51%至 98%。对于临床分离的细菌，生物膜抑制率为 6%至 60%。因此，本研究表明橙花叔醇具有抗氧化、抗菌和抗生物膜特性。
