Department of Surgery, Second Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, P.R. China.
Int J Oncol. 2010 Jun;36(6):1401-8.
The aim of this study was to elucidate the role of CD97 isoforms in gastric carcinoma. Out of four gastric cancer cell lines investigated, BGC-823 cells demonstrating low CD97 protein expression were stably transfected with pcDNA3.1 vector containing CD97/EGF1,2,5 or CD97/EGF1,2,3,4,5 inserts. Behavior of transfected cells was systematically investigated by employing proliferation, motility and invasive assays. As a result, we found that over-expression of CD97/EGF1,2,5 isoform correlated with increased motile and invasive ability of the clones. Furthermore, CD97/EGF1,2,5 isoform over-expression (3.8 times higher) was followed by significant decrease of CD97/EGF1,2,3,4,5 isoform (10.3 times lower). In contrast, CD97/EGF1,2,3,4,5 clones revealed significantly reduced invasive properties as compared with corresponding controls. The changes in acetylation status were one of the possible mechanisms affecting behavior of transfected cells. We concluded from the study that CD97 is closely related with advanced stages and higher invasiveness of gastric carcinoma. The study further lightened the tumor promoting role of CD97 small isoform in cancer progression and indicated the possible suppressive properties of the full length isoform of CD97.
本研究旨在阐明 CD97 异构体在胃癌中的作用。在研究的四种胃癌细胞系中，BGC-823 细胞中 CD97 蛋白表达较低，通过稳定转染 pcDNA3.1 载体，该载体含有 CD97/EGF1、2、5 或 CD97/EGF1、2、3、4、5 插入物，研究人员对其进行了研究。通过增殖、迁移和侵袭实验系统地研究了转染细胞的行为。结果发现，CD97/EGF1、2、5 异构体的过表达与克隆的迁移和侵袭能力增强相关。此外，CD97/EGF1、2、5 异构体的过表达（高 3.8 倍）伴随着 CD97/EGF1、2、3、4、5 异构体的显著减少（低 10.3 倍）。相比之下，CD97/EGF1、2、3、4、5 克隆的侵袭特性明显低于相应的对照。乙酰化状态的变化是影响转染细胞行为的可能机制之一。我们从研究中得出结论，CD97 与胃癌的晚期和高侵袭性密切相关。该研究进一步强调了 CD97 小异构体在癌症进展中的促肿瘤作用，并表明 CD97 全长异构体可能具有抑制作用。
