Sanchis R, Pardon P, Abadie G
CNEVA, Laboratoire de Pathologie, Biot, France.
Ann Rech Vet. 1991;22(1):59-64.
The aim of the experiment was to evaluate in pregnant ewes the clinical, bacteriological and serological effects of conjunctival instillation of S abortusovis. An individual dose of 1 x 10(10) viable S abortusovis were administered to 20 ewes 92 d after mating. Among 18 pregnant ewes, 8 aborted 110 +/- 13 d after mating, 4 had stillborn lambs and 6 a clinically normal lambing (114 +/- 1.3 d). S abortusovis was isolated from placentas, swabs and foetuses of the 12 lambings. Evolution of "H" agglutination titers measured by means of a microtechnique using a coloured antigen was found to be independent of the clinical results. Instillation by conjunctival route could constitute an experimental model closer to a natural mode of infection than the subcutaneous route.
该实验的目的是评估给怀孕母羊结膜滴注绵羊流产布鲁氏菌后的临床、细菌学和血清学效应。在交配92天后，给20只母羊分别接种1×10¹⁰个活的绵羊流产布鲁氏菌。18只怀孕母羊中，8只在交配后110±13天流产，4只产出死胎羔羊，6只产羔临床正常（114±1.3天）。在12次产羔的胎盘、拭子和胎儿中分离出绵羊流产布鲁氏菌。通过使用有色抗原的微量技术测定的“H”凝集效价的变化与临床结果无关。与皮下途径相比，结膜途径滴注可能构成更接近自然感染方式的实验模型。
