United States Department of Agriculture, Agricultural Research Service, U.S. Horticultural Research Laboratory, 2001 South Rock Road, Fort Pierce, FL 34945, USA.
J Econ Entomol. 2010 Apr;103(2):541-9. doi: 10.1603/ec09360.
Sampling statistics were obtained to develop a sampling protocol for estimating numbers of adult Diaphorina citri Kuwayama (Hemiptera: Psyllidae) in citrus by using two different sampling methods: yellow sticky traps and stem-tap samples. A 4.0-ha block of mature orange trees was stratified into 100.4-ha strata and sampled using each method seven times over a 7-mo period. One sticky trap was deployed per tree on each of 16 trees randomly selected in each stratum, and numbers of adults on the traps were counted 1 wk later. One stem-tap sample in which the number of adults falling into a pan after three rapid taps to a branch was taken per tree on each of 16 trees randomly selected in each stratum. A sampling protocol of one yellow sticky trap on each of 20 trees, or of one stem-tap sample on each of 30 trees, distributed uniformly across an area up to 4.0 ha (excluding block edges) was projected to provide an average sampling precision rate of < or = 25% (SEM/mean x 100) at means of one or more adults per trap or stem-tap sample. Validation sampling indicated 20 sticky trap samples consistently provided the desired precision level at means of approximately two or more adults per trap but not at means of 1.0-1.5 per trap. A sample size of 30 stem-tap samples consistently provided the desired average precision level, but the precision of some individual estimates was > 25% at means of around one adult per tap sample.
黄色诱捕器和茎干敲击采样法,对柑橘成年柑橘木虱(半翅目:木虱科)数量进行估计的采样方案进行了采样统计。在一个 4.0 公顷的成熟橘树种植区内,将其分为 100.4 公顷的区,然后用这两种方法在 7 个月的时间内对每个区进行 7 次采样。在每个区的随机选择的 16 棵树中的每棵树上,都放置了一个黄色诱捕器,一周后统计诱捕器上的成虫数量。在每个区的随机选择的 16 棵树中的每棵树上,通过快速敲击三次树枝,将掉入收集盘的成虫数量进行计数。在每个区的随机选择的 16 棵树中的每棵树上,都进行一次茎干敲击采样,每次敲击后收集落入收集盘的成虫数量。预测方案是在 4.0 公顷的范围内(不包括区块边缘),均匀分布 20 棵树上的一个黄色诱捕器或 30 棵树上的一个茎干敲击采样器,每次采样预计会有一个或更多的成虫,这一方案的平均采样精度为 <或= 25%(SEM/mean x 100)。验证采样表明,20 个诱捕器样本每次采样有大约 2 个或更多成虫时,可保持预期的精度水平,但当每次采样有 1.0-1.5 个成虫时,精度则无法达到预期水平。30 个茎干敲击采样器样本的采样方案每次采样都能保持预期的平均精度水平,但在每次采样大约有一个成虫时,部分样本的精度 > 25%。