Snyder James, Dickens Katrina L, Halbert Susan E, Dowling Stefanie, Russell Dyrana, Henderson Ruth, Rohrig Eric, Ramadugu Chandrika
Florida Department of Agriculture and Consumer Services, Division of Plant Industry, Gainesville, FL 32608, USA.
USDA APHIS PPQ S&T, Salinas, CA 93905, USA.
Insects. 2022 Mar 16;13(3):295. doi: 10.3390/insects13030295.
Citrus huanglongbing (HLB) is a severe problem for citrus cultivation. The disease management programs benefit from improved field tools suitable for surveying the ACP vector (Asian citrus psyllid, Kuwayama (Hemiptera: Psyllidae)) and the associated pathogen. In the present study, we utilize three-dimensional (3D) printers and design tools to develop traps that can capture and preserve ACPs. Three novel, 3D-printed traps were designed and evaluated: stem trap, and cylinder traps 1 and 2. The traps and yellow sticky cards were deployed weekly for 8 months in 2 non-commercial citrus groves in Florida; in California, the traps were evaluated for 12 months in field cages and 4 citrus groves. The stem traps captured lower numbers of ACPs at all experimental sites compared to the cylinder traps. Capture rates in the cylinder traps were comparable to the sticky trap, making the device a viable tool for monitoring field ACPs. The two main advantages of using the reusable 3D traps over standard methods of ACP and HLB surveys include dynamic sampling that can be conducted year-round and the capture of ACPs that can be preserved and tested. Improved trapping may facilitate quick management decisions and mitigate HLB.
柑橘黄龙病（HLB）是柑橘种植面临的一个严重问题。病害管理计划受益于适用于调查柑橘木虱传播媒介（亚洲柑橘木虱，Kuwayama（半翅目：木虱科））及相关病原体的改良田间工具。在本研究中，我们利用三维（3D）打印机和设计工具开发了能够捕获并保存亚洲柑橘木虱的诱捕器。设计并评估了三种新型3D打印诱捕器：茎干诱捕器、圆筒诱捕器1和圆筒诱捕器2。在佛罗里达州的2个非商业柑橘园中，诱捕器和黄色粘虫板每周部署一次，持续8个月；在加利福尼亚州，诱捕器在田间笼子和4个柑橘园中进行了12个月的评估。与圆筒诱捕器相比，茎干诱捕器在所有试验地点捕获的亚洲柑橘木虱数量都较少。圆筒诱捕器的捕获率与粘虫板相当，这使得该装置成为监测田间亚洲柑橘木虱的可行工具。与亚洲柑橘木虱和柑橘黄龙病调查的标准方法相比，使用可重复使用的3D诱捕器的两个主要优点包括可全年进行动态采样以及能够捕获可保存和检测的亚洲柑橘木虱。改进诱捕方法可能有助于快速做出管理决策并减轻柑橘黄龙病的危害。
