核共振振动光谱法鉴定蛋白质结合的亚硝酰铁复合物。
Identification of protein-bound dinitrosyl iron complexes by nuclear resonance vibrational spectroscopy.
机构信息
Department of Chemistry, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA.
出版信息
J Am Chem Soc. 2010 May 26;132(20):6914-6. doi: 10.1021/ja101002f.
Abstract
We have applied (57)Fe nuclear resonance vibrational spectroscopy (NRVS) to identify protein-bound dinitrosyl iron complexes. Intense NRVS peaks due to vibrations of the N-Fe-N unit can be observed between 500 and 700 cm(-1) and are diagnostic indicators of the type of iron dinitrosyl species present. NRVS spectra for four iron dinitrosyl model compounds are presented and used as benchmarks for the identification of species formed in the reaction of Pyrococcus furiosus ferredoxin D14C with nitric oxide.
摘要
我们已经应用（57）Fe 核共振振动光谱（NRVS）来鉴定蛋白质结合的双亚硝酰铁配合物。在 500 到 700 厘米（-1）之间，可以观察到由于 N-Fe-N 单元振动而产生的强烈 NRVS 峰，这是存在的铁双亚硝酰物种类型的诊断指标。本文介绍了四种铁双亚硝酰模型化合物的 NRVS 谱，并将其用作鉴定 Pyrococcus furiosus 铁氧还蛋白 D14C 与一氧化氮反应中形成的物种的基准。
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