Department of Neurology, University of Turku, Finland.
Scand J Clin Lab Invest. 2010 Jul;70(4):275-80. doi: 10.3109/00365511003792975.
To study the effects of rapid i.v. glucose bolus on insulin, leptin, ghrelin, peptide YY (PYY), free fatty acids (FFA), glucagon and glucagon-like peptide-1 (GLP-1) concentrations together with self-reported satiety ratings in lean and obese human subjects.
Twenty-five healthy subjects were recruited, 12 were lean (mean age = 26 years, BMI range = 19.8-23.9 kg/m(2)) and 13 were obese (mean age = 27 years, BMI range = 27.7-42.2 kg/m(2)). In two separate 55 min counter-balanced blinded sessions (separate days), subjects were administered an i.v. dose of 300 mg/kg glucose or saline. Blood concentrations of several feeding-related hormones were recorded at multiple time points, together with ratings of satiety and euphoria.
Greater increases in glucose concentrations were observed in the obese group compared to the lean group (p < 0.0001). In both lean and obese subjects, glucose injection induced a clear fall in the concentrations of FFA, ghrelin, glucagon and PYY (p < 0.0001) but not in the concentrations of leptin or GLP-1. Obese subjects showed positive correlations between satiety and glucose, but only at time points 30 min (r = 0.73, p = 0.005) and 55 min (r = 0.82, p = 0.0005).
The directions and the magnitudes of short-term hormonal changes after i.v. glucose challenge are the same in lean and moderately obese subjects. Possible short-term regulatory effects of leptin and GLP-1 can not be induced by acute energy load bypassing the GI-tract.
研究快速静脉注射葡萄糖对瘦人和肥胖人体中胰岛素、瘦素、胃饥饿素、肽 YY（PYY）、游离脂肪酸（FFA）、胰高血糖素和胰高血糖素样肽-1（GLP-1）浓度以及自我报告的饱腹感评分的影响。
招募了 25 名健康受试者，其中 12 名为瘦人（平均年龄=26 岁，BMI 范围为 19.8-23.9kg/m²），13 名为肥胖者（平均年龄=27 岁，BMI 范围为 27.7-42.2kg/m²）。在两个单独的 55 分钟对照盲法实验中（在不同的日子），给受试者静脉注射 300mg/kg 的葡萄糖或生理盐水。在多个时间点记录了几种与进食相关的激素的血浓度，并对饱腹感和欣快感进行了评分。
与瘦人组相比，肥胖组的血糖浓度升高更明显（p<0.0001）。在瘦人和肥胖受试者中，葡萄糖注射均导致 FFA、胃饥饿素、胰高血糖素和 PYY 的浓度明显下降（p<0.0001），但瘦素和 GLP-1 的浓度没有下降。肥胖受试者的饱腹感与血糖呈正相关，但仅在 30 分钟（r=0.73，p=0.005）和 55 分钟（r=0.82，p=0.0005）时间点有相关性。
静脉注射葡萄糖后短期激素变化的方向和幅度在瘦人和中度肥胖者中是相同的。通过绕过胃肠道的急性能量负荷，瘦素和 GLP-1 的可能短期调节作用不能被诱导。
