松茸中一种新型多糖的结构解析及抗氧化活性研究。
Structure elucidation and antioxidant activity of a novel polysaccharide isolated from Tricholoma matsutake.
机构信息
Key Laboratory of Biological Resource and Ecological Environment of the Ministry of Education, College of Life Sciences, Sichuan University, Chengdu 610064, PR China.
出版信息
Int J Biol Macromol. 2010 Aug 1;47(2):271-5. doi: 10.1016/j.ijbiomac.2010.04.010. Epub 2010 Apr 27.
In this study, structural features of Tricholoma matsutake polysaccharide (TMP-A) were investigated by a combination of infrared (IR) spectra, gas chromatography-mass spectrometry (GC-MS), nuclear magnetic resonance (NMR) spectroscopy. The results indicated that TMP-A had a backbone of 1,4-beta-d-glucopyranose residue which branches at O-6 based on the experimental results. The branches were mainly composed of an (1-->3)-alpha-d-galactopyranose residue, and terminated with alpha-d-xylopyranose residue. The antioxidant activity of TMP-A was evaluated with several biochemical methods, including DPPH(-) radical scavenging, hydrogen peroxide scavenging, superoxide anion radical scavenging. The results indicated that TMP-A showed strong antioxidant. In the in vitro antioxidant assay by MTT method, TMP-A could attenuate PC12 cell damage significantly caused by hydrogen peroxide.
在这项研究中，通过红外（IR）光谱、气相色谱-质谱（GC-MS）、核磁共振（NMR）光谱的综合分析，研究了松茸多糖（TMP-A）的结构特征。实验结果表明，TMP-A 的主链由 1,4-β-d-吡喃葡萄糖残基组成，基于实验结果，该主链在 O-6 处分支。支链主要由（1-->3）-α-d-半乳糖吡喃糖残基组成，并以α-d-木吡喃糖残基结尾。采用几种生化方法评价了 TMP-A 的抗氧化活性，包括 DPPH（-）自由基清除、过氧化氢清除、超氧阴离子自由基清除。结果表明，TMP-A 表现出很强的抗氧化活性。在 MTT 法体外抗氧化试验中，TMP-A 可显著减轻由过氧化氢引起的 PC12 细胞损伤。
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