从红绒盖牛肝菌中分离得到的一种新型多糖的结构解析及抗氧化活性研究
Structure elucidation and antioxidant activity of a novel polysaccharide isolated from Boletus speciosus Forst.
机构信息
Key Laboratory of Southwest China Wildlife Resources Conservation, College of Life Sciences, China West Normal University, 1# Shida Road, Nanchong, Sichuan Province 637009, China.
出版信息
Int J Biol Macromol. 2012 Apr 1;50(3):613-8. doi: 10.1016/j.ijbiomac.2012.01.021. Epub 2012 Jan 20.
In this study, a novel heteropolysaccharide was isolated from the fruiting bodies of Boletus speciosus Forst through DEAE-cellulose column and Sephadex G-200 column. The Boletus speciosus Forst polysaccharide (BSFP-1) had a molecular weight of 1.33×10(4) Da and was mainly composed of l-Man and d-Gal which ratios were 2:1. Structural features of Boletus speciosus Forst polysaccharide (BSFP-1) were investigated by a combination of total hydrolysis, methylation analysis, gas chromatography-mass spectrometry (GC-MS), infrared (IR) spectra and nuclear magnetic resonance (NMR) spectroscopy. The results indicated that Boletus speciosus Forst polysaccharide (BSFP-1) had a backbone of (1→4)-α-l-mannopyranose residues which branches at O-6 based on the experimental results. The branches were mainly composed of one with →1)-α-d-galactopyranose residue. The antioxidant activity of BSFP-1 was evaluated with two biochemical methods, including 1,1-diphenyl-2-picrylhydrazyl (DPPH(-)) radical scavenging, scavenging activity of 2,2'-azino-bis(3-ethylbenzthiazoline-6-suphonic acid)diammonium (ABTS(+)) radical. The results indicated that BSFP-1 showed strong antioxidant.
在这项研究中，通过 DEAE-纤维素柱和 Sephadex G-200 柱从华丽牛肝菌子实体中分离出一种新型杂多糖。华丽牛肝菌多糖（BSFP-1）的分子量为 1.33×10(4) Da，主要由 l-Man 和 d-Gal 组成，比例为 2:1。通过完全水解、甲基化分析、气相色谱-质谱联用（GC-MS）、红外（IR）光谱和核磁共振（NMR）光谱的组合，研究了华丽牛肝菌多糖（BSFP-1）的结构特征。结果表明，华丽牛肝菌多糖（BSFP-1）具有（1→4）-α-l-甘露吡喃糖残基的主链，根据实验结果，该主链在 O-6 处分支。支链主要由一个 →1）-α-d-半乳糖吡喃糖残基组成。通过两种生化方法评估 BSFP-1 的抗氧化活性，包括 1,1-二苯基-2-苦基肼（DPPH(-)）自由基清除、2,2'-联氮双（3-乙基苯并噻唑啉-6-磺酸）二铵盐（ABTS(+)）自由基清除活性。结果表明，BSFP-1 具有较强的抗氧化活性。
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