Department of Pharmacology, School of Chemical Biology and Pharmaceutical Sciences, Capital Medical University, Youanmen, Beijing 100069, PR China.
J Pharm Biomed Anal. 2010 Nov 2;53(3):698-704. doi: 10.1016/j.jpba.2010.03.041. Epub 2010 Apr 8.
A sensitive and specific liquid chromatography-tandem mass spectrometry method (LC-MS) was developed and validated for the separation and simultaneous determination of danshensu, rosmarinic acid and tanshinone compounds including cryptotanshinone, tanshinone I, dihydrotanshinone I and tanshinone IIA in rat plasma. Chromatographic separation of the analytes was successfully achieved on a C(18) column using a mobile phase composed of acetonitrile-water containing 0.5% glacial acetic acid. This method demonstrated good linearity and did not have endogenous material interfering with the active compounds and I.S. peaks. The limit of quantification of danshensu, rosmarinic acid, cryptotanshinone, dihydrotanshinone I, tanshinone I and tanshinone IIA were 5, 0.75, 0.1, 0.1, 1 and 0.5 ng/mL. The average extraction recoveries of these analytes from rat plasma were all over 60%. The precisions determined from five days were all within 10%. This method has been successfully applied in the simultaneous quantification and the pharmacokinetic studies of these six compounds in animals which were orally administered with danshen preparations.
建立并验证了一种灵敏、特异的液相色谱-串联质谱法（LC-MS），用于分离和同时测定大鼠血浆中的丹酚酸、迷迭香酸和丹参酮类化合物，包括丹参素、迷迭香酸、隐丹参酮、丹参酮 I、二氢丹参酮 I 和丹参酮 IIA。采用含 0.5%冰醋酸的乙腈-水作为流动相，在 C18 柱上成功实现了分析物的色谱分离。该方法具有良好的线性，无内源性物质干扰活性化合物和内标峰。丹参素、迷迭香酸、隐丹参酮、二氢丹参酮 I、丹参酮 I 和丹参酮 IIA 的定量下限分别为 5、0.75、0.1、0.1、1 和 0.5ng/mL。这些分析物从大鼠血浆中的平均提取回收率均超过 60%。5 天内测定的精密度均在 10%以内。该方法已成功应用于口服丹参制剂后动物体内这 6 种化合物的同时定量和药代动力学研究。
