Modern Research Center for Traditional Chinese Medicine, School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100029, China.
Department of pharmacy, Baotou Medical College, Baotou 014060, China.
Molecules. 2017 Oct 30;22(11):1853. doi: 10.3390/molecules22111853.
An attempt was made to characterize the pharmacokinetic profiles of (QSKL) that has been widely proved to be effective in clinical practice. A method using ultra-high performance liquid chromatography coupled with tandem mass spectrometry (UHPLC-MS/MS) for the simultaneous determination of 25 analytes in rat plasma was developed and validated. Satisfactory chromatographic separation was achieved on an ACQUITY UPLC HSS T3 column with gradient elution using mobile phase consisting of 0.02% aqueous formic acid (A) and acetonitrile fortified with 0.02% formic acid (B), and analyte detection was carried out using polarity-switching multiple reaction monitoring mode. Method validation assays in terms of selectivity, linearity, inter- and intra-day variations, matrix effect, and recovery demonstrated the newly developed method to be specific, sensitive, accurate, and precise. Following the oral administration of QSKL at a single dose, the qualified method was successfully applied for pharmacokinetic investigations in sham and model rats. Mild differences occurred for the pharmacokinetic patterns of most components between those two groups, whereas significant differences were observed for glycyrrhizic acid and glycyrrhetic acid. The obtained findings could provide meaningful information for the clarification of the effective material basis of QSKL.
尝试对已被广泛证明在临床实践中有效的（QSKL）的药代动力学特征进行表征。开发并验证了一种使用超高效液相色谱-串联质谱联用（UHPLC-MS/MS）同时测定大鼠血浆中 25 种分析物的方法。在 ACQUITY UPLC HSS T3 柱上，使用含有 0.02%甲酸的水相（A）和乙腈（B）作为流动相进行梯度洗脱，实现了令人满意的色谱分离，并采用极性切换多反应监测模式进行分析物检测。选择性、线性、日内和日间变异、基质效应和回收率等方法验证试验表明，新开发的方法具有特异性、灵敏度、准确性和精密度。在单次口服 QSKL 后，该合格方法成功应用于假手术和模型大鼠的药代动力学研究。两组之间大多数成分的药代动力学模式存在轻微差异，而甘草酸和甘草次酸则存在显著差异。所得结果可为阐明 QSKL 的有效物质基础提供有意义的信息。
