体育锻炼对糖尿病大鼠免疫系统的影响。
Effects of physical training on the immune system in diabetic rats.
作者信息
Crespilho Daniel Maciel, de Almeida Leme José Alexandre Curiacos, de Mello Maria Alice Rostom, Luciano Eliete
机构信息
Department of Physical Education, São Paulo State University (UNESP), Rio Claro-SP, Brazil.
出版信息
Int J Diabetes Dev Ctries. 2010 Jan;30(1):33-7. doi: 10.4103/0973-3930.60010.
AIMS
This study aims to investigate the influence of physical training on the immune system of diabetic rats.
MATERIALS AND METHODS
Adult male Wistar rats were distributed into Sedentary Control (SC), Trained Control (TC), Sedentary Diabetic (SD) and Trained Diabetic (TD) groups were used. Diabetes was induced by alloxan (32 mg/bw-i.v.). Training protocol consisted of swimming, at 32 +/- 1 degrees C, one hour/day, five days/week, supporting an overload equivalent to 5% of the body weight, during four weeks. At the end of the experiment the rats were sacrificed by decapitation and blood samples were collected for glucose, insulin, albumin, hematocrit determinations, total and differential leukocyte counting. Additionally, liver samples for glycogen analyses were obtained.
RESULTS
The results were analyzed by one way at a significance level of 5%. Diabetes reduced blood insulin, liver glycogen stores and increased blood glucose and neutrophil count. Physical training restored glycemia, liver glycogen levels, neutrophils and lymphocytes count in diabetic rats.
CONCLUSIONS
In summary, physical training was able to improve metabolic and immunological aspects in the experimental diabetic rats.
目的
本研究旨在调查体育锻炼对糖尿病大鼠免疫系统的影响。
材料与方法
将成年雄性Wistar大鼠分为久坐对照组（SC）、训练对照组（TC）、久坐糖尿病组（SD）和训练糖尿病组（TD）。用四氧嘧啶（32mg/体重-静脉注射）诱导糖尿病。训练方案包括在32±1℃下游泳，每天1小时，每周5天，持续四周，负荷相当于体重的5%。实验结束时，断头处死大鼠，采集血样进行血糖、胰岛素、白蛋白、血细胞比容测定，以及白细胞总数和分类计数。此外，获取肝脏样本进行糖原分析。
结果
结果采用单因素分析，显著性水平为5%。糖尿病降低了血液胰岛素、肝脏糖原储备，增加了血糖和中性粒细胞计数。体育锻炼恢复了糖尿病大鼠的血糖、肝脏糖原水平、中性粒细胞和淋巴细胞计数。
结论
总之，体育锻炼能够改善实验性糖尿病大鼠的代谢和免疫状况。
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