• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

嗜热链球菌中链霉菌胆固醇氧化酶基因的转移与表达

Transfer and expression of a Streptomyces cholesterol oxidase gene in Streptococcus thermophilus.

作者信息

Somkuti G A, Solaiman D K, Johnson T L, Steinberg D H

机构信息

U.S. Department of Agriculture, ARS, Eastern Regional Research Center, Philadelphia, Pennsylvania 19118.

出版信息

Biotechnol Appl Biochem. 1991 Apr;13(2):238-45.

PMID:2043280
Abstract

The recombinant plasmid pNCO937 (8.1 kbp) containing a Streptomyces sp. cholesterol oxidase gene was introduced into Streptococcus thermophilus by electrotransformation. Transformation frequency was 7.2 x 10(5) colony forming units/micrograms of DNA. The presence of the cholesterol oxidase gene in S. thermophilus was confirmed with Southern blot analysis using a biotinylated probe. Thin-layer chromatographic analysis showed the expression of the Streptomyces cholesterol oxidase gene resulting in the oxidation of cholesterol to 4-cholesten-3-one. S. thermophilus may be a suitable host for the expression of other genes regulating prokaryotic cholesterol metabolism.

摘要

含有链霉菌属胆固醇氧化酶基因的重组质粒pNCO937（8.1千碱基对）通过电转化法导入嗜热链球菌。转化频率为7.2×10⁵菌落形成单位/微克DNA。使用生物素化探针通过Southern印迹分析证实了嗜热链球菌中胆固醇氧化酶基因的存在。薄层色谱分析表明链霉菌胆固醇氧化酶基因的表达导致胆固醇氧化为4-胆甾烯-3-酮。嗜热链球菌可能是表达其他调节原核生物胆固醇代谢基因的合适宿主。

相似文献

1
Transfer and expression of a Streptomyces cholesterol oxidase gene in Streptococcus thermophilus.嗜热链球菌中链霉菌胆固醇氧化酶基因的转移与表达
Biotechnol Appl Biochem. 1991 Apr;13(2):238-45.
2
Native promoter-plasmid vector system for heterologous cholesterol oxidase synthesis in Streptococcus thermophilus.用于嗜热链球菌中异源胆固醇氧化酶合成的天然启动子-质粒载体系统。
Plasmid. 1995 Jan;33(1):7-14. doi: 10.1006/plas.1995.1002.
3
Expression of Streptomyces melC and choA genes by a cloned Streptococcus thermophilus promoter STP2201.通过克隆的嗜热链球菌启动子STP2201表达链霉菌melC和choA基因。
J Ind Microbiol. 1995 Jul;15(1):39-44. doi: 10.1007/BF01570011.
4
Cloning and expression of a Streptomyces cholesterol oxidase gene in Streptomyces lividans with plasmid pIJ702.利用质粒pIJ702在变铅青链霉菌中克隆和表达链霉菌胆固醇氧化酶基因。
Appl Environ Microbiol. 1986 Dec;52(6):1382-5. doi: 10.1128/aem.52.6.1382-1385.1986.
5
Cholesterol oxidase ChoL is a critical enzyme that catalyzes the conversion of diosgenin to 4-ene-3-keto steroids in Streptomyces virginiae IBL-14.胆固醇氧化酶 ChoL 是一种关键酶，可催化薯蓣皂素在变铅青链霉菌 IBL-14 中转化为 4-烯-3-酮甾体。
Appl Microbiol Biotechnol. 2010 Feb;85(6):1831-8. doi: 10.1007/s00253-009-2188-0. Epub 2009 Aug 27.
6
Construction and characterization of shuttle plasmids for lactic acid bacteria and Escherichia coli.用于乳酸菌和大肠杆菌的穿梭质粒的构建与表征
Plasmid. 1992 Jul;28(1):25-36. doi: 10.1016/0147-619x(92)90033-7.
7
Cloning of the gene for cholesterol oxidase in Bacillus spp., Lactobacillus reuteri and its expression in Escherichia coli.芽孢杆菌属、罗伊氏乳杆菌中胆固醇氧化酶基因的克隆及其在大肠杆菌中的表达。
Lett Appl Microbiol. 1993 Aug;17(2):61-4. doi: 10.1111/j.1472-765x.1993.tb00371.x.
8
Hyperexpression and analysis of choB encoding cholesterol oxidase of Brevibacterium sterolicum in Escherichia coli and Streptomyces lividans.短小芽孢杆菌胆固醇氧化酶编码基因choB在大肠杆菌和变铅青链霉菌中的高效表达及分析
Biosci Biotechnol Biochem. 1992 Nov;56(11):1786-91. doi: 10.1271/bbb.56.1786.
9
[Cloning of the Streptococcus thermophilus beta-galactosidase gene and its expression in Escherichia coli and Bacillus subtilis cells].嗜热链球菌β-半乳糖苷酶基因的克隆及其在大肠杆菌和枯草芽孢杆菌细胞中的表达
Mol Gen Mikrobiol Virusol. 1990 Apr(4):10-4.
10
Shuttle vectors developed from Streptococcus thermophilus native plasmid.由嗜热链球菌天然质粒构建的穿梭载体。
Plasmid. 1993 Jul;30(1):67-78. doi: 10.1006/plas.1993.1034.

引用本文的文献

1
Expression optimization, purification, and functional characterization of cholesterol oxidase from Chromobacterium sp. DS1.从鞘氨醇单胞菌 DS1 中表达优化、纯化和功能表征胆固醇氧化酶。
PLoS One. 2019 Feb 13;14(2):e0212217. doi: 10.1371/journal.pone.0212217. eCollection 2019.
2
Expression optimization of recombinant cholesterol oxidase in Escherichia coli and its purification and characterization.重组胆固醇氧化酶在大肠杆菌中的表达优化及其纯化与表征
AMB Express. 2018 Nov 12;8(1):183. doi: 10.1186/s13568-018-0711-3.
3
The naturally competent strain Streptococcus thermophilus LMD-9 as a new tool to anchor heterologous proteins on the cell surface.
热链球菌 LMD-9 作为一种新的工具，可将异源蛋白锚定在细胞表面。
Microb Cell Fact. 2014 Jun 5;13:82. doi: 10.1186/1475-2859-13-82.
4
Expression of a Lactococcus lactis Phage Resistance Mechanism by Streptococcus thermophilus.通过嗜热链球菌表达乳球菌噬菌体抗性机制。
Appl Environ Microbiol. 1995 Jul;61(7):2461-6. doi: 10.1128/aem.61.7.2461-2466.1995.
5
Rhodococcus erythropolis ATCC 25544 as a suitable source of cholesterol oxidase: cell-linked and extracellular enzyme synthesis, purification and concentration.红平红球菌ATCC 25544作为胆固醇氧化酶的合适来源：细胞结合型和胞外酶的合成、纯化及浓缩
BMC Biotechnol. 2002 Mar 26;2:3. doi: 10.1186/1472-6750-2-3.
6
Identification and mutagenesis by allelic exchange of choE, encoding a cholesterol oxidase from the intracellular pathogen Rhodococcus equi.通过等位基因交换对编码来自细胞内病原体马红球菌的胆固醇氧化酶的choE进行鉴定和诱变。
J Bacteriol. 2001 Aug;183(16):4796-805. doi: 10.1128/JB.183.16.4796-4805.2001.
7
Expression of Streptomyces melC and choA genes by a cloned Streptococcus thermophilus promoter STP2201.通过克隆的嗜热链球菌启动子STP2201表达链霉菌melC和choA基因。
J Ind Microbiol. 1995 Jul;15(1):39-44. doi: 10.1007/BF01570011.
8
Expression of cho and melC operons by a Streptococcus thermophilus synthetic promoter in Escherichia coli.嗜热链球菌合成启动子在大肠杆菌中对cho和melC操纵子的表达。
Appl Microbiol Biotechnol. 1995 May-Jun;43(2):285-90. doi: 10.1007/BF00172826.