Brigidi P, Bolognani F, Rossi M, Cerre C, Matteuzzi D
Institute of Pharmaceutical and Toxicological Chemistry, University of Catania, Italy.
Lett Appl Microbiol. 1993 Aug;17(2):61-4. doi: 10.1111/j.1472-765x.1993.tb00371.x.
The cloning of the cholesterol oxidase gene in several Gram-positive bacteria, including Lactobacillus reuteri of intestinal origin, was obtained. Only the transformants of Escherichia coli harbouring the recombinant plasmid pCHOA showed a good intracellular enzyme activity. The heterologous gene was stably maintained in Gram-positive transformants but no enzyme activity was detected.
已成功克隆了几种革兰氏阳性细菌中的胆固醇氧化酶基因，其中包括源于肠道的罗伊氏乳杆菌。只有携带重组质粒pCHOA的大肠杆菌转化子表现出良好的细胞内酶活性。该异源基因在革兰氏阳性转化子中稳定维持，但未检测到酶活性。
