Unitat d'Histologia i Neurobiologia (UHN), Facultat de Medicina i Ciències de la Salut, Universitat Rovira i Virgili, Reus, Spain.
J Peripher Nerv Syst. 2010 Mar;15(1):40-9. doi: 10.1111/j.1529-8027.2010.00250.x.
Neurotrophins and their receptors, the trk receptor tyrosine kinases (trks) and p75(NTR), are differentially expressed among the cell types that make up synapses. It is important to determine the precise location of these molecules involved in neurotransmission. Here we use immunostaining and Western blotting to study the localization and expression of neurotrophin brain-derived neurotrophic factor (BDNF) and neurotrophin-4 (NT-4) and the receptors tropomyosin-related kinase b (trkB) and p75(NTR) at the adult neuromuscular junction. Our confocal immunofluorescence results on the whole mounts of the mouse Levator auris longus muscle and on semithin cross-sections showed that BDNF, NT-4, trkB, and p75(NTR) were localized on the three cells in the neuromuscular synapse (motor axons, post-synaptic muscle and Schwann cells).
神经营养因子及其受体，即酪氨酸激酶受体 trk（trks）和 p75（NTR），在构成突触的细胞类型中呈现不同的表达。确定参与神经传递的这些分子的精确位置非常重要。在这里，我们使用免疫染色和 Western blot 来研究成年神经肌肉接头中脑源性神经营养因子（BDNF）和神经营养因子-4（NT-4）以及受体原肌球蛋白相关激酶 b（trkB）和 p75（NTR）的定位和表达。我们对小鼠镫骨肌整体标本和半薄横切的共聚焦免疫荧光结果表明，BDNF、NT-4、trkB 和 p75（NTR）定位于神经肌肉突触的三个细胞上（运动轴突、突触后肌肉和雪旺细胞）。
