使用连接介导的 PCR（HELP）进行 HpaII 微小片段富集的 DNA 甲基化分析。
DNA methylation profiling using HpaII tiny fragment enrichment by ligation-mediated PCR (HELP).
机构信息
Center for Epigenomics and Division of Computational Genetics, Department of Genetics, Albert Einstein College of Medicine, 1301 Morris Park Avenue, Bronx, NY 10461, USA.
出版信息
Methods. 2010 Nov;52(3):218-22. doi: 10.1016/j.ymeth.2010.04.013. Epub 2010 Apr 29.
Abstract
The HELP assay is a technique that allows genome-wide analysis of cytosine methylation. Here we describe the assay, its relative strengths and weaknesses, and the transition of the assay from a microarray to massively-parallel sequencing-based foundation.
摘要
HELP assay 是一种能够进行全基因组胞嘧啶甲基化分析的技术。在这里，我们描述了该技术，包括其相对优势和劣势，以及该技术从微阵列到大规模平行测序基础的转变。
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