来自硬毛藻的新型荧光蛋白具有氰基发射。

Novel fluorescent protein from Hydnophora rigida possess cyano emission.

机构信息

Department of Environmental Science and Policy, Microbiome Analysis Center, George Mason University, Manassas, VA, USA.

出版信息

Biochem Biophys Res Commun. 2010 Jun 4;396(3):631-6. doi: 10.1016/j.bbrc.2010.04.136. Epub 2010 May 7.

Abstract

Currently, a broad diversity of fluorescent proteins among marine organisms range from cyano-red emissions. Fluorescent proteins differ in their DNA sequences from green fluorescent protein (GFP). We identified cDNA encoding the gene of a new protein from the reef coral Hydnophora rigida of the Merulinidae family. Both the spectral properties and putative primary sequence of the protein has been determined. The cloned cDNA encode peptide we call HriCFP is comprised of 134 amino acids. It has characteristics of a cyano fluorescent protein (HriCFP) and its sequence is markedly different from known GFP from the hydroid jellyfish Aequorea victoria. HriCFP was cloned, expressed, purified and exist as monomer. The peptide mass finger print on the purified protein confirmed identity of HriCFP.

摘要

目前,海洋生物中的荧光蛋白种类繁多,从氰基红色发射光到绿色荧光蛋白(GFP)不等。荧光蛋白在 DNA 序列上与绿色荧光蛋白(GFP)不同。我们从 Merulinidae 家族的石珊瑚 Hydnophora rigida 中鉴定出一种新蛋白基因的 cDNA。该蛋白的光谱特性和假定的一级序列已被确定。克隆的 cDNA 编码我们称之为 HriCFP 的肽由 134 个氨基酸组成。它具有氰基荧光蛋白(HriCFP)的特征,其序列与水螅水母 Aequorea victoria 的已知 GFP 明显不同。HriCFP 已被克隆、表达、纯化并以单体形式存在。纯化蛋白的肽质量指纹图谱证实了 HriCFP 的身份。

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