Ianushevich Iu G, Gurskaia N G, Staroverov D B, Luk'ianov S A, Luk'ianov K A
Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, ul. Miklukho-Maklaya 16/10, GSP Moscow, 117997 Russia.
Bioorg Khim. 2003 Jul-Aug;29(4):356-60.
The gene of a new red fluorescent protein zoan2RFP from a coral polyp Zoanthus sp., a homologue of the known green fluorescent protein from the Aequorea victoria jellyfish, was cloned. At early maturation stages, zoan2RFP exhibits a green fluorescence, which then turns into the red one. A similar phenomenon was recently reported for the E5 mutant of the red fluorescent coral protein DsRed. Zoan2RFP differs from E5 by faster maturation kinetics and the complete disappearance of green fluorescence in the mature protein. Naturally occurring proteins of this type can be considered as intermediate forms between the green and red fluorescent proteins, which are formed during the microevolution of fluorescent proteins.
从珊瑚虫类动物纽扣珊瑚（Zoanthus sp.）中克隆出一种新的红色荧光蛋白zoan2RFP的基因，它是维多利亚多管水母已知绿色荧光蛋白的同源物。在早期成熟阶段，zoan2RFP呈现绿色荧光，随后转变为红色荧光。最近有报道称红色荧光珊瑚蛋白DsRed的E5突变体也有类似现象。zoan2RFP与E5的不同之处在于其成熟动力学更快，且成熟蛋白中的绿色荧光完全消失。这种天然存在的蛋白质可被视为绿色和红色荧光蛋白之间的中间形式，它们是在荧光蛋白的微进化过程中形成的。
