Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Mahidol University, 447 Sri-Ayudhaya Rd, Rajathevee, Bangkok 10400, Thailand.
Anal Bioanal Chem. 2010 Sep;398(1):29-52. doi: 10.1007/s00216-010-3741-5. Epub 2010 May 2.
This review covers recent advances of capillary electrophoresis (CE) in pharmaceutical analysis. The principle, instrumentation, and conventional modes of CE are briefly discussed. Advances in the different CE techniques (non-aqueous CE, microemulsion electrokinetic chromatography, capillary isotachophoresis, capillary electrochromatography, and immunoaffinity CE), detection techniques (mass spectrometry, light-emitting diode, fluorescence, chemiluminescence, and contactless conductivity), on-line sample pretreatment (flow injection) and chiral separation are described. Applications of CE to assay of active pharmaceutical ingredients (APIs), drug impurity testing, chiral drug separation, and determination of APIs in biological fluids published from 2008 to 2009 are tabulated.
本文综述了近年来毛细管电泳（CE）在药物分析中的新进展。简要讨论了 CE 的原理、仪器和常规模式。此外，还介绍了不同 CE 技术（非水相 CE、微乳液电动色谱、胶束电动毛细管色谱、毛细管电色谱和免疫亲和 CE）、检测技术（质谱、发光二极管、荧光、化学发光和非接触式电导）、在线样品预处理（流动注射）和手性分离的进展。本文还列举了 2008 年至 2009 年期间发表的 CE 在测定原料药（API）、药物杂质检测、手性药物分离和生物体液中 API 测定中的应用。
