Veterinary Medicine Faculty, Department of Veterinary Morphophisiology and Animal Production (DIMORFIPA), University of Bologna, Bologna, Italy.
Vet Res Commun. 2010 Jun;34 Suppl 1:S21-4. doi: 10.1007/s11259-010-9408-z.
Endothelin (ET)-1 is a potent vasoconstrictor peptide involved in the derangement of respiratory mechanics during endotoxic shock. We measured the kinetics of pulmonary mRNA expression of the key components of the ET system [i.e., ET-1, ET-converting enzyme (ECE), and ETA and ETB receptors] by quantitative real-time reverse transcriptase-polymerase chain reaction in a swine model of endotoxic shock (0, 1, 2, 3, and 4 h of continuous LPS infusion at 40 microg/kg/hour; sham group, 4 hour saline infusion). A significant increase in mRNA expression levels was observed for ET-1 in LPS-treated piglets; the increase began as early as 1 hour. In contrast, no significant variations were observed for the ECE, ETA, or ETB genes. Small gene expression differences observed with respect to our previous results suggest a possible effect of the anesthesia or surgical protocol on ET system regulation.
内皮素（ET）-1 是一种强效的血管收缩肽，参与内毒素休克时呼吸力学的紊乱。我们通过定量实时逆转录聚合酶链反应测量了内毒素休克猪模型中 ET 系统的关键成分（即 ET-1、ET 转化酶（ECE）和 ETA 和 ETB 受体）的肺 mRNA 表达动力学（持续 LPS 输注 4 小时，每小时 40 微克/千克；假手术组，4 小时生理盐水输注）。在 LPS 处理的仔猪中，观察到 ET-1 的 mRNA 表达水平显著增加；这种增加早在 1 小时就开始了。相比之下，ECE、ETA 或 ETB 基因没有观察到显著的变化。与我们之前的结果相比，观察到的基因表达差异较小，这表明麻醉或手术方案可能对内毒素系统的调节有影响。
