De Massis F, Bonfini B, Zaghini M, Tittarelli M
Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise 'G. Caporale' (IZS A&M), Campo Boario, Teramo, Italy.
Vet Ital. 2005 Apr-Jun;41(2):90-6.
An indirect ELISA was developed by the Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise 'G. Caporale' (IZS A&M) for the detection of Brucella antibodies in cow's milk. Specific monoclonal antibody was used against a bovine IgG(1) epitope and complies with European Commission requirements. The test accuracy was evaluated on milk samples from the regions of Abruzzo and Molise in Italy. The negative samples came from 1,250 officially brucellosis-free herds from the Molise region (Italy). The positive samples were taken from three herds in the Abruzzo and Molise regions where animals positive to the official tests were present and Brucella abortus was isolated. Test specificity was 99.8% (with a confidence interval [CI] of 99.6%-99.9%), while sensitivity was 100% (CI 91.2%-100%). The probability of detecting antibodies in positive milk samples was higher than 50% up to a dilution of 1:256 in negative milk. The probability of identifying an infected herd in the dairy cattle population. Under study was 88.6% (CI 73.9%-95.3%).
阿布鲁佐和莫利塞大区动物卫生实验研究所“G. 卡波拉尔”（IZS A&M）开发了一种间接酶联免疫吸附测定法（ELISA），用于检测牛奶中的布鲁氏菌抗体。使用了针对牛IgG(1)表位的特异性单克隆抗体，该抗体符合欧盟委员会的要求。对意大利阿布鲁佐和莫利塞地区的牛奶样本进行了检测准确性评估。阴性样本来自意大利莫利塞地区1250个官方无布鲁氏菌病的牛群。阳性样本取自阿布鲁佐和莫利塞地区的三个牛群，这些牛群中有动物经官方检测呈阳性且分离出了流产布鲁氏菌。检测特异性为99.8%（置信区间[CI]为99.6%-99.9%），而敏感性为100%（CI 91.2%-100%）。在阴性牛奶中，阳性牛奶样本稀释至1:256时检测到抗体的概率高于50%。在所研究的奶牛群体中识别受感染牛群的概率为88.6%（CI 73.9%-95.3%）。
