Paolini A, Ridolfi V, Zezza D, Cocchietto M, Musa M, Pavone A, Conte A, Giorgetti G
Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise 'G. Caporale' (IZS A&M), Teramo, Italy.
Vet Ital. 2005 Apr-Jun;41(2):137-44.
Photobacterium damsela subsp. piscicida (Phdp) is the aetiological agent of fish pasteurellosis, causing heavy losses in intensive mariculture plants. The present work compares the protective efficacy of five different vaccine formulation: oral, intraperitoneal, immersion, bivalent immersion (Vibrio anguillarum) and immersion associated with immunostimulants. Each of these vaccine formulations containing whole cells of Phdp formalin inactivated (FKC), was administered to 100 sea bass weighing approximately 2 g; 100 non-vaccinated sea bass were used as controls. Protection against pasteurellosis was tested for 40 days after vaccination by intraperitoneal challenge: each fish was inoculated with Phdp cells at a concentration of 2.75 x 10(4) cfu/ml. Mortality was recorded over the following 14 days, vaccine protection was evaluated using a relative percentage survival (RPS) index. The intraperitoneal formulation gave excellent protection (RPS 82.4%). The most effective immersion form was that followed by simple immersion (RPS 23.1%) followed by the group vaccinated with bivalent vaccine (RPS 18.7%). Protection conferred orally (RPS 28.6%) is of interest for practical purposes.
杀鲑气单胞菌杀鲑亚种（Phdp）是鱼类巴氏杆菌病的病原体，给集约化海水养殖厂造成重大损失。本研究比较了五种不同疫苗制剂的保护效果：口服、腹腔注射、浸泡、二价浸泡（鳗弧菌）以及与免疫刺激剂联合浸泡。每种疫苗制剂均含有福尔马林灭活的Phdp全细胞（FKC），将其施用于100尾体重约2 g的海鲈；100尾未接种疫苗的海鲈用作对照。接种疫苗40天后，通过腹腔注射攻毒来测试对巴氏杆菌病的保护作用：每尾鱼接种浓度为2.75×10⁴ cfu/ml的Phdp细胞。在接下来的14天记录死亡率，使用相对存活率（RPS）指数评估疫苗保护效果。腹腔注射制剂提供了极佳的保护效果（RPS 82.4%）。最有效的浸泡方式是单纯浸泡（RPS 23.1%），其次是接种二价疫苗的组（RPS 18.7%）。口服给予的保护作用（RPS 28.6%）在实际应用中具有重要意义。
